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Stable transformation of lettuce cultivar South Bay from cotyledon explants

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Abstract

Transgenic plants of lettuce cultivar (cv.) ‘South Bay’ were produced by using Agrobacterium tumefaciens vectors containing the β-glucuronidase (GUS) reporter gene and the NPT II gene for kanamycin resistance as a selectable marker. High frequency of transformation, based on kanamycin resistance and assays for GUS expression, was obtained with 24 to 72-h-old cotyledon explants cocultivated for 48 h with Agrobacterium tumefaciens. After the cocultivation period, the explants were placed in selection medium containing 50 or 100 mg l−1 of kanamycin, 100 mg l−1 cefotaxime and 500 mg l−1 carbenicillin for 10 days. Surviving explants were transferred every 14 days on shoot elongation medium. Progenies of R0 plants demonstrated linked monogenic segregation for kanamycin resistance and GUS activity.

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Author notes

  1. A. C. Torres

    Present address: EMBRAPA/CNPH, P.O. Box 07-0218, 70359, Brasilia, DF, Brazil

  2. M. Ashraf

    Present address: Plant Molecular Biology Center, Northern Illinois University, 60115, DeKalb, IL, USA

Authors and Affiliations

  1. Vegetable Crops Department, University of Florida, IFAS, P.O. Box 110690, 32611-0690, Gainesville, FL, USA

    A. C. Torres, D. J. Cantliffe, B. Laughner, M. Bienick, R. Nagata, M. Ashraf & R. J. Ferl

Authors
  1. A. C. Torres
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  2. D. J. Cantliffe
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  3. B. Laughner
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  4. M. Bienick
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  5. R. Nagata
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  6. M. Ashraf
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  7. R. J. Ferl
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Additional information

Florida Agricultural Experiment Station Journal Series R-02231. This research was partially supported by CNPq/RHAE (Brazil).

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Torres, A.C., Cantliffe, D.J., Laughner, B. et al. Stable transformation of lettuce cultivar South Bay from cotyledon explants. Plant Cell Tiss Organ Cult 34, 279–285 (1993). https://doi.org/10.1007/BF00029717

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  • DOI: https://doi.org/10.1007/BF00029717

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