Abstract
We studied the influence of temperature and near- and sub- optimal mineral nutrition of black spruce seedlings (Picea mariana [Mill.] B.S.P.) during their second growing period on bud set, bud development, growth, mineral content and cold tolerance. Bud break and growth after bud break were also studied. Seedlings were grown for 106 d in growth chambers under three temperature regimes in combination with three concentrations of a fertilizer. They were then cold hardened for 56 d and dehardened for 66 d.
Under these near- and sub-optimal N levels, bud formation occurred during the growing season. Bud formation was accelerated with decreasing fertilization, but was not affected by temperature treatments. Needles from seedlings with 0.64% N (dry mass basis) before hardening did not harden. Those with 0.87% N showed a lesser degree of hardiness than those with 1.28% N. Stem diameter increased at the beginning of the hardening period. During this acclimation period, shoot dry mass decreased with time at a constant rate and at the same rate over time for all treatments whereas root dry mass was more variable. Total number of needle primordia was low and no difference was observed among growing conditions. Bud break was similar in all treatments. Following bud break, shoot height and stem diameter increases were small but their magnitude varied with the nutritional regimes applied during the previous growing period. During hardening, nitrogen concentration of shoot tissues first increased and then decreased; phosphorus concentration first increased and then remained stable; potassium concentration remained stable. Concentration of these three elements generally decreased in the roots during this hardening.
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References
Anonymous 1984. Deuxième atelier de travail sur la culture des semis en récipients. Service canadien des forêts. Ministère de l'Énergie et des Ressources du Québec. Ordre des ingénieurs forestiers du Québec. Sainte-Foy (QC), 29–30 nov. 139 pp.
Anonymous 1990. Troisième atelier québécois sur la culture des plants forestiers en récipients. Gouv. du Québec, ministère de l'Énergie et des Ressources (forêts). Direction de la recherche et du développement. Service du transfert de technologie. 341 pp.
Benzian, B., Brown, R.M. and Freeman, S.C.R. 1974. Effect of late-season top-dressings of N (and K) applied to conifer transplants in the nursery on their survival and growth on British forest sites. Forestry 47: 153–184.
Bigras, F.J. 1987. Influence de la température, de la photopériode et de la nutrition minérale sur l'acclimatation et la tolérance au gel de Juniperus chinensis “Pfitzerana” et de Juniperus chinensis “Pfitzerana aurea”. Ph.D. thesis. Université Laval. Québec (QC). 153 pp.
Bigras, F.J. and D'Aoust, A.L. 1992. Hardening and dehardening of shoots and roots of containerized black spruce and white spruce seedlings under short and long days. Can. J. For. Res. 22: 388–396.
Bittenbender, H.C. and Howell, G.S.Jr. 1974. Adaptation of the Spearman-Kärber method for estimating the T50 of cold stressed flower buds. J. Am. Soc. Hort. Sci. 99: 187–190.
Bremner, J.M. and Mulvaney, C.S. 1982. Nitrogen-total, pp. 595–624. In: Page, A.L., Miller, R.H. and Keeney, D.R. (Eds) Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties, Vol. 9. Agron. Monogr. (Madison).
Chapman, H.D. and Pratt, P.E. 1961. Methods of Analysis for Soils, Plants and Waters. University of California, Division of Agricultural Science, Riverside, Calif. 309 pp.
Christersson, L. 1975. Frost hardiness development in rapid- and slow-growing Norway spruce seedlings. Can. J. For. Res. 5: 340–343.
Colombo, S.J., Webb, D.P. and Glerum, C. 1982. Cold hardiness and bud development under short days in black spruce and white spruce seedlings, pp. 171–176. In: Scarratt, J.B., Glerum, C. and Plexman, C.A. (Eds) Proceedings, Canadian Containerized Tree Seedling Symposium, Canadian Forest Service, Great Lakes Forestry Centre, COJFRC Symp. Proc. O-P-10.
Cox, D.R. 1970. Analysis of Binary Data. Methuen, London, 142 pp.
Dormling, I. and Gustafsson, Å. and von, Wettstein, D. 1968. The experimental control of the life cycle in Picea abies (L.) Karst. I. Some basic experiments on the vegetative cycle. Silvae Genet. 17: 44–64.
Langlois, C.-G. 1990. Le dynamisme de la croissance des plants cultivés dans le récipient 45–110 et les relations entre les variables, pp. 1–24. In. Troisième atelier québécois sur la culture des plants forestiers en récipients. Gouv. du Québec, ministère de l'Énergie et des Ressources (forêts). Direction de la recherche et du développement. Service du transfert de technologie.
Levitt, J. 1956. The Hardiness of Plants. Academic Press, N.Y. 278 pp.
Levitt, J. 1980. Responses of Plants to Environmental Stresses. Vol. 1. Chilling, Freezing, and High Temperature Stresses. Academic Press, New York. 497 pp.
Macey, D.E. and Arnott, J.T. 1986. The effect of moderate moisture and nutrient stress on bud formation and growth of container-grown white spruce seedlings. Can. J. For. Res. 16: 949–954.
Margolis, H.A. and Brand, D.G. 1990. An ecophysiological basis for understanding plantation establishment. Can. J. For. Res. 20: 375–390.
McGregor, W.H.D. and Kramer, P.J. 1963. Seasonal trends in rates of photosynthesis and respiration of loblolly pine. Am. J. Bot. 50: 760–765.
Milliken, G.A. and Johnson, D.E. 1984. Analysis of messy data. Vol. 1: Designed experiments. Van Nostrand Reinhold, New York. 473 pp.
Olsen, S.R. and Sommers, L.E. 1982. Phosphorus, pp. 403–430. In: Page, A.L., Miller, R.H. and Keeney, D.R. (Eds) Methods of Soil Analysis, Part 2. Chemical and Microbiological Properties, Vol. 9. Agron. Monogr. (Madison).
Owston, P.W. and Kozlowski, T.T. 1981. Growth and cold hardiness of container-grown Douglas-fir, noble fir, and Sitka spruce seedlings in simulated greenhouses regimes. Can. J. For. Res. 4: 97–100.
Pellett, H.M. and Carter, J.V. 1981. Effect of nutritional factors on cold hardiness of plants. Hort. Rev. 3: 144–171.
Pellett, N.E. and White, D.B. 1969. Effect of soil nitrogen and soil moisture levels on the cold acclimation of container grown Juniperus chinensis “Hetzi”. J. Am. Soc. Hort. Sci. 94: 457–459.
Pollard, D.F.W. 1974. Seedling size and age as factors of morphogenesis in white spruce Picea glauca (Moench) Voss buds. Can. J. For. Res. 4: 97–100.
Rowell, J.G. and Walters, D.E. 1976. Analysing data with repeated observations on each experimental unit. J. Agric. Sci. 87: 423–432.
Sandvik, M. 1980. Environmental control of winter stress tolerance and growth potential in seedlings of Picea abies (L.) Karst. Part B. Use of controlled environment facilities. N.Z. J. For. Sci. 10: 97–104.
SAS Institute, Inc. 1990. SAS/Stat User's Manual. Volume 2. Carey, North Carolina.
Swan, H.S.D. 1965. Studies of the mineral nutrition of Canadian pulpwood species. Phase II. Fertilizer pellet field trials. 1959–1963. Final report. Pulp and Paper Research Institute of Canada. Tech. Rep., 1965, 405.
Templeton, C.W.G., Odlum, K.D. and Colombo, S.J. 1991. How to Dissect Spruce Buds. Ontario Ministry of Natural Resources, Sault Ste. Marie, Ontario.
van den, Driessche, R. 1991. Effects of nutrients on stock performance in the forest, pp. 229–260. In: van den, Driessche, R. (Ed) Mineral Nutrition of Conifer Seedlings. CRC Press, Boca Raton, Florida.
Young, E. and Hanover, J.W. 1977. Development of the shoot apex of blue spruce (Picea pungens). Can. J. For. Res. 7: 614–620.
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Bigras, F.J., Gonzalez, A., D'Aoust, A.L. et al. Frost hardiness, bud phenology and growth of containerized Picea mariana seedlings grown at three nitrogen levels and three temperature regimes. New Forest 12, 243–259 (1996). https://doi.org/10.1007/BF00027934
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DOI: https://doi.org/10.1007/BF00027934