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Range of photosynthetic control of postillumination P700+ reduction rate in sunflower leaves

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Abstract

The kinetics of the postillumination reduction of P700+ which reflects the rate constant for plastoquinol (PQH2) oxidation was recorded in sunflower leaves at different photon absorption densities (PAD), CO2 and O2 concentrations. The P700 oxidation state was calculated from the leaf transmittance at 830 nm logged at 50 μs intervals. The P700+ dark reduction kinetics were fitted with two exponents with time constants of 6.5 and about 45 ms at atmospheric CO2 and O2 concentrations. The time constant of the fast component, which is the major contributor to the linear electron transport rate (ETR), did not change over the range of PADs of 14.5 to 134 nmol cm-2 s-1 in 21% O2, but it increased up to 40 ms under severe limitation of ETR at low O2 and CO2. The acceptor side of Photosystem I (PS I) became reduced in correlation with the downregulation of the PQH2 oxidation rate constant. It is concluded that thylakoid pH-related downregulation of the PQH2 oxidation rate constant (photosynthetic control) is not present under normal atmospheric conditions but appears under severe limitation of the availability of electron acceptors. The measured range of photosynthetic control fits with the maximum variation of ETR under natural stress in C3 plants. Increasing the carboxylase/oxygenase specificity would lead to higher reduction of the PS I acceptor side under stress.

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Abbreviations

Cyt b 6 f :

cytochrome b 6 f complex

Cw :

cell-wall CO2 concentration, μM

ETR:

electron transport rate

Fd:

ferredoxin

FNR:

ferredoxin-NADP reductase

FRL:

far-red light

PC:

plastocyanin

PAD:

photon absorption density nmol cm-2 s-1

PFD:

photon flux density nmol cm-2 s-1

PS I:

Photosystem I complex

PQ:

plastoquinon

PQH2 :

plastoquinol

PS II:

Photosystem II complex

P700:

Photosystem I donor pigment, reduced

S830:

830 nm signal (D830, difference of S830 from the dark level)

WL:

white light

Yl :

maximum quantum yield of PS I electron transport, rel. un

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Authors and Affiliations

  1. Institute of Molecular and Cell Biology, Tartu University, Riia str. 181, EE2400, Tartu, Estonia

    Agu Laisk & Vello Oja

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  1. Agu Laisk
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  2. Vello Oja
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Laisk, A., Oja, V. Range of photosynthetic control of postillumination P700+ reduction rate in sunflower leaves. Photosynth Res 39, 39–50 (1994). https://doi.org/10.1007/BF00027141

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  • DOI: https://doi.org/10.1007/BF00027141

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