Abstract
A new and efficient method for clonal propagation of Casuarina sumatrana by rooting stem cuttings is described. High percentage (about 60–70%) of rooting was achieved with mature softwood stem cuttings. A quick-dip of 5 s in NAA (1–10mM) solution followed by sand culture under high humidity were required for a high rate of survival and rooting of stem cuttings. A simple, closed chamber propagation system, using fluorocarbon polymer (tetrafluoroethyleneperfluoroalkyl vinyl ether) film (Neoflon PFA film), was successfully developed for the rooting of stem cuttings without mist. Rooted cuttings inoculated with Frankia were easily transplanted and established in field conditions with very low (about 3%) mortality. The significance of these findings for mass clonal propagation of C. sumatrana is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- IBA:
-
indole-3-butyric acid
- NAA:
-
α-naphthaleneacetic acid
References
Burggraaf AJP and Shipton WA (1982) Estimates of Frankia growth under various pH and temperature regimes. Plant Soil 69: 135–147
Duhoux E, Sougoufara B and Dommergues Y (1986) Propagation of Casuarian equisetifolia through axillary buds of immature female inflorescences cultured in vitro. Plant Cell Rep 3: 161–164
Hartmann HT, Kester DE and Davis FT (1990) Plant Propagation. Principles and Practices, Fifth edition, p 645. New Jersey: Prentice-Hall International Editions, New Jersey
Hussain S (1957) A note on vegetative multiplication of Casuarina cnninghammi. Indian For 83: 226–227
Husain AMM and Ponnuswamy PK (1980) Propagation of Casuarina junghuhniana by planting shoots and root suckers. Indian For 106: 298–299
Lundquist R and Torrey JG (1984) The propagation of Casuarina species from rooted stem cutting. Bot Gaz 145: 378–384
Midgley SJ, Turnbull W and Johnston RD (eds) (1983) Casuarina Ecology, Management and Utilization, p 286. Melboume: CSIRO
National Academy of Sciences (1980) Firewood Crops, p 237. Washington, DC: National Academy Press
National Academy of Sciences (1984) Casuarinas; Nitrogenfixing Trees for Adverse Sites, p 118. Washington, DC: National Academy Press
Paton DM, Willing RR, Nichols W and Pryor LD (1970) Rooting of stem cuttings of Eucalyptus: a rooting inhibitor in addult tissue. Aust J Bot 18: 175–183
Somasundaram TR and Jagadees SS (1977) Propagation of Casuarina equisetifolia Forst. by planting shoots. Indian For 103: 737–738
Tanaka M (1991) Disposable film culture vessels. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 17 High-Tech and Micropropagation 1, pp 212–228. Berlin: Springer-Verlag
Torrey JG (1983) Root development and root nodulation in Casuarina. In: Midgley SJ, Turnbull W and Johnston RD (eds) Casuarina Ecology, Management and Utilization, pp 180–192. Melbourne: CSIRO
Turnbull JW and Martensz PN (1983) Seed production, collection and germination in Casuarinaceae. In: Midgley SJ, Turnbull W and Johnston RD (eds) Casuarina Ecology, Management and Utilization, pp 126–132. Melbourn: CSIRO Melbourn
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goh, C.J., Lakshmanan, P., Lee, C.L. et al. A simple and efficient method for clonal propagation of Casuarina sumatrana (de Vriese) L. Johnson. Plant Growth Regul 17, 115–120 (1995). https://doi.org/10.1007/BF00024170
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00024170