Summary
Plantlets of a diploid species, Rosa wichuraiana, were cultured in vitro in liquid medium and treated with spindle inhibitors or tritiated thymidine. Cytological studies were then carried out on cells of root tips which were excised after the treatments. Exposure to 0.05% colchicine gave complete spindle arrest with less toxicity than 0.1% colchicine. Solutions of colcemid gave incomplete spindle arrest even at highly toxic concentrations. After a 0.5 h pulse of 3H thymidine, the periodicity in the curve of percentage of labelled mitoses against time indicated a cell-cycle-time of 10 h. The highest frequency of tetraploid cells was obtained after exposure to 0.05% colchicine for 12 h and it is suggested that colchicine prolonged the cell-cycle-time by 2 h. Seven days after a 12 h exposure to 0.05% colchicine, 32.7% of cells in the root meristem were tetraploid and it is anticipated that a satisfactory yield of non-chimeral tetraploids could be regenerated from such tissues.
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Roberts, A.V., Lloyd, D. & Short, K.C. In vitro procedures for the induction of tetraploidy in a diploid rose. Euphytica 49, 33–38 (1990). https://doi.org/10.1007/BF00024128
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DOI: https://doi.org/10.1007/BF00024128