Summary
Resistance to Plasmodiophora brassicae Wor. race 7, the causal agent of the disease clubroot, was examined in an F2 population of a cross between a clubroot resistant broccoli (Brassica oleracea var. italica) and a susceptible cauliflower (B. oleracea var. botrytis). A genetic linkage map was constructed in the same population based on the segregation of 58 dispersed restriction fragment length polymorphism (RFLP) markers. Associations between the inheritance of RFLP marker genotypes and segregation for disease resistance, morphological and maturity characteristics were examined. For each triat examined, several chromosomal regions marked by RFLP probes appeared to contain trait loci, suggesting that each trait was under polygenic control. RFLP marker linkage to a major factor imparting dominance for clubroot resistance from the broccoli parent was observed in this population. Additionally, RFLP marker linkage to an independently segregating factor contributing clubroot resistance from the cauliflower parent was observed, indicating that it should be possible to use RFLP markers to facilitate selection of transgressive segregants having the combined resistance from both parental sources. In some instances, RFLP markers from the same or closely linked chromosomal regions were associated with both clubroot resistance and morphological traits. Analysis of RFLP marker genotypes at linked loci should facilitate the selection of desired disease resistant morphotypes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baggett, J.R., 1976. ‘Oregon Cr-1’ broccoli. HortScience 11: 622–623.
Baggett, J.R., 1983. Clubroot-resistant cabbage breeding lines Oregon 100, 123, 140, and 142. HortScience 18(1): 112–114.
Baggett, J.R. & D. Kean, 1985. Clubroot resistant broccoli breeding lines OSU CR-2 to OSU CR-8. HortScience 20: 784–485.
Buczacki, S.T., 1979. Plasmodiophora brassicae. CMI descriptions of pathogenic fungi and bacteria, No. 621. The Commonwealth Mycological Institute, Kew Surrey.
Chiang, M.S. & R. Crête, 1970. Inheritance of clubroot resistance in cabbage (Brassica oleracea L. var. capitata L.). Can. J. Genet. Cytol. 12: 253–256.
Chiang, M.S. & R. Crête, 1972. Screening crucifers for germplasm resistance to clubroot Plasmodiophora brassicae. Can. Plant. Dis. Surv. 52(2): 45–50.
Chiang, M.S. & R. Crête, 1976. Diallel analysis of the inheritance of resistance to race 6 of Plasmodiophora brassicae in cabbage. Can. J. Plant Sci. 56: 865–868.
Chiang, M.S. & R. Crête, 1983. Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea ssp. capitata). V. The inheritance of resistance. Euphytica 32: 479–483.
Crisp, P., I.R. Crute, R.A. Sutherland, S.M. Angeli, K. Bloor, H. Burgess & P. Gordon, 1989. The exploitation of genetic resources of Brassica oleracea in breeding for resistance to clubroot (Plasmodiophora brassicae). Euphytica 42: 215–226.
Crute, I.R., A.R. Gray, P. Crisp & S.T. Buczacki, 1980. Variation in Plasmodiophora brassicae and resistance to clubroot disease in Brassicas and allied crops—a critical review. Plant Breed. Abstr. 50: 91–104.
Crute, I.R., K. Phelps, A. Barnes, S.T. Buczacki & P. Crisp, 1983. The relationship between genotypes of three Brassica species and collections of Plasmodiophora brassicae. Plant Pathology 32: 405–420.
Edwards, M.D., C.W. Stuber & J.F. Wendel, 1987. Molecular marker facilitated investigations of quantitative trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 16: 113–125.
Ferreira, M.E., J. Robak & P.H. Williams, 1989. Filter paper disc method for clubroot screening. Proc. Fifth Crucifer Genetics Workshop. University of California, Davis.
Figdore, S.S., W.C. Kennard, K.M. Song, M.K. Slocum & T.C. Osborn, 1988. Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor. Appl. Genet. 75: 833–840.
Guffy, R.D., C.W. Stuber & M.D. Edwards, 1989. Dissecting and enhancing heterosis in corn using molecular markers. Twenty-fifth Annual Illinois Corn Breeders School. pp. 99–119. University of Illinois, Champaign.
Helentjaris, T., 1987. A genetic linkage map for maize based on RFLPs. Trends Genet. 3: 217–221.
Helentjaris, T., G. King, M. Slocum, C. Siedenstrang & S. Wegman, 1985. Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol. Biol. 5: 109–118.
Helentjaris, T., M. Slocum, S. Wright, A. Schaefer & J. Nienhuis, 1986. Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor. Appl. Genet. 72: 761–769.
Jacobsen, B.J. & P.H. Williams, 1970. Control of cabbage clubroot using benomyl fungicide. Plant Dis. Rep. 54: 456–460.
Kennard, W.C., K.M. Song, S.S. Figdore, K. Suzuki, M.K. Slocum & T.C. Osborn, 1988. Genetic linkage maps and phenotypic trait analysis in Brassica using molecular markers. XVIth Intl. Cong. Genet., Ottawa Canada.
Landry, B.S., R.V. Kesseli, B. Farrara & R.W. Michelmore, 1987. A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance, and morphological markers. Genetics 116: 331–337.
Landry, B.S., N. Hubert, T. Etoh, J.J. Harada & S.E. Lincoln 1991. A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34(4): 543–552.
Nienhuis, J., T. Helentjaris, M. Slocum, B. Ruggero & A. Schaefer, 1987. Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci. 27: 707–803.
Nieuwhof, M. & D. Wiering, 1962. Clubroot resistance in Brassica oleracea L. Euphytica 11: 233–239.
Osborn, T.C., D.C. Alexander & J.F. Fobes, 1987. Identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor. Appl. Genet. 73: 784–794.
Paterson, A.H., E.S. Lander, J.D. Hewitt, S. Peterson, S.E. Lincoln & S.D. Tanksley, 1988. Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335: 721–726.
Saghai-Maroof, M., K. Soliman, R. Jorgensen & R. Allard, 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci., USA 81: 8014–8018.
Slocum, M.K., S.S. Figdore, W.C. Kennard, J.Y. Suzuki & T.C. Osborn, 1990. Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor. Appl. Genet. 80: 57–64.
Song, K.M., J.Y. Suzuki, M.K. Slocum, P.H. Williams & T.C. Osborn, 1991. A linkage map of Brassica rapa (syn. campestris) based on restriction fragment length polymorphism loci. Theor. Appl. Genet. 82: 296–304.
Steel, R.G.D. & J.H. Torrie, 1980. Principles and Procedures of Statistics Second ed. McGraw-Hill, Inc: New York, 633p.
Tanksley, S.D. & J. Hewitt, 1988. Use of molecular markers in breeding for soluble solids content in tomato—a re-examination. Theor. Appl. Genet. 75: 811–823.
Vriesenga, J.D. & S. Honma, 1971. Inheritance of seedling resistance to clubroot in Brassica oleracea L. HortScience 6: 395–396.
Weisaeth, G., 1974. Some problems and results in breeding for clubroot resistance in cole crops. Proc. Eucarpia Meetings-Cruciferae. pp. 101–107.
Walker, J.C. & R.H. Larson, 1951. Progress in the development of clubroot-resistant cabbage. Phytopathology 41: 37.
Walker, J.C. & R.H. Larson, 1960. Development of the first clubroot-resistant cabbage variety. Wisconsin Agric. Expt. Stat. Bull. 547: 12–16.
Weller, J.I., M. Soller & T. Brody, 1988. Linkage analysis of quantitative traits in an interspecific cross of tomato (Lycopersicon esculentum x Lycopersicon pimpinellifolium) by means of genetic markers. Genetics 118: 329–339.
White, J.G. & S.T. Buczacki, 1977. Control of clubroot by soil partial sterilisation with particular reference to the treatment of small natural foci of infection. Ann. Appl. Biol. 87: 337–343.
Williams, P.H., 1966. A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56: 624–626.
Williams, P.H., 1985. Crucifer Genetics Coop. Resource Book. Dept. of Plant Pathology. University of Wisconsin, Madison.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Figdore, S.S., Ferreira, M.E., Slocum, M.K. et al. Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L.. Euphytica 69, 33–44 (1993). https://doi.org/10.1007/BF00021723
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00021723