Abstract
A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein could be synthesized in E. coli. The properties of this PEPC protein clearly resembled those of typical C3 plant enzymes.
Similar content being viewed by others
References
Andreo, CS, Gonzales, DH, Iglesias, AA: Higher plant phosphoenolpyruvate carboxylase. FEBS Lett 213: 1–8 (1987).
Anraku, Y: The reduction and restoration of galactose transport in osmotically shocked cells of Escherichia coli. J Biol Chem 242: 793–800 (1967).
Bakrim, N, Echevarria, C, Cretin, C, Arrio-Dupont, M, Pierre, JN, Vidal, J, Chollet, R, Gadal, P: Regulatory phosphorylation of Sorghum leaf phosphoenolpyruvate carboxylase: identification of the protein-serine kinase and some elements of the signal transduction cascade. Eur J Biochem 204: 821–830 (1992).
Bradford, M: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).
Cretin, C, Bakrim, N, Keryer, E, Santi, S, Lepiniec, L, Vidal, J, Gadal, P: Production in Escherichia coli of active Sorghum phosphoenolpyruvate carboxylase which can be phosphorylated. Plant Mol Biol 17: 83–88 (1991).
Cushman, JC, Bohnert, HJ: Nucleotide sequence of the Ppc2 gene encoding a housekeeping isoform of phosphoenolpyruvate carboxylase from Mesembryanthemum crystallinum. Nucl Acids Res 17: 6743–6744 (1989).
Denecke M: Reinigung und Charakterisierung der stomatären Phosphoenolpyruvat-Carboxylase aus Vicia faba L. Entwicklung eines geeigneten Verfahrens zur Herstellung monoklonaler Antikörper. Dissertation, University of Bonn, FRG (1991).
Eikmanns, BJ, Follettie, MT, Griot, MU, Sinskey, AJ: The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucloetide sequence, and expression. Mol Gen Genet 218: 330–339 (1989).
Hermans, J, Westhoff, P: Homologous genes for the C4 isoforms of phosphoenolpyruvate carboxylase in a C3 and a C4 Flaveria species. Mol Gen Genet 234: 275–284 (1992).
Izui, K, Ishijima, S, Yamaguchi, Y, Katagiri, F, Murata, T, Shigesada, K, Sugiyama, T, Katsuki, F: Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C4-pathway from maize. Nucl Acids Res 14: 1615–1628 (1986).
Jiao, JA, Vidal, J, Echevarria, C, Chollet, R: In vivo regulatory phosphorylation site in C4 leaf phosphoenolpyruvate carboxylase from maize and sorghum. Plant Physiol 96: 297–301 (1991).
Koizumi, N, Sato, F, Terano, Y, Yamada, Y: Sequence analysis of cDNA encoding phosphoenolpyruvate carboxylase from cultured tobacco cells. Plant Mol Biol 17: 535–539 (1991).
Latzko, E, Kelly, GJ: The many-faceted function of phosphoenolpyruvate carboxylase in C3 plants. Physiol Vég 21: 805–815 (1983).
Lepiniec, L, Keryer, E, Philippe, H, Gadal, P, Cretin, C: Sorghum phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution. Plant Mol Biol 21: 487–502 (1993).
O'Leary, MH: Phosphoenolpyruvate carboxylase: an enzymologist's view. Annu Rev Plant Physiol 33: 297–315 (1982).
Poetsch, W, Hermans, J, Westhoff, P: Multiple cDNAs of phosphoenolpyruvate carboxylase in the C4 dicot Flaveria trinervia. FEBS Lett 292: 133–136 (1991).
Rickers, J, Cushman, JC, Michalowski, CB, Schmitt, JM, Bohnert, HJ: Expression of the CAM form of phosphoenolpyruvate carboxylase and nucleotide sequence of a full length cDNA from Mesembryanthemum crystallinum Mol Gen Genet 215: 447–454 (1989).
Sabe, H, Miwa, T, Kodaki, T, Izui, K, Hiraga, S, Katsuki, H: Molecular cloning of the phosphoenolpyruvate carboxylase gene, ppc, of Escherichia coli. Gene 31: 279–283 (1984).
Schnabl, H, Denecke, M, Schulz, M: In vitro and in vivo phosphorylation of stomatal phosphoenolpyruvate carboxylase from Vicia faba L. Bot Acta 105: 367–369 (1992).
Sugimoto, T, Kawasaki, T, Kato, T, Whittier, RF, Shibata, D, Kawamura, Y: cDNA sequence and expression of a phosphoenolpyruvate carboxylase gene from soybean. Plant Mol Biol 20: 743–747 (1992).
Ting, IP, Osmond, CB: Photosynthetic phosphoenolpyruvate carboxylases. Characteristics of alloenzymes from leaves of C3 and C4 plants. Plant Physiol 51: 439–447 (1973).
Yanagisawa, S, Izui, K, Yamaguchi, Y, Shigesada, K, Katsuki, H: Further analysis of cDNA clones for maize phosphoenolpyruvate carboxylase involved in C4 photosynthesis. FEBS Lett 229: 107–110 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Merkelbach, S., Gehlen, J., Denecke, M. et al. Cloning, sequence analysis and expression of a cDNA encoding active phosphoenolpyruvate carboxylase of the C3 plant Solanum tuberosum . Plant Mol Biol 23, 881–888 (1993). https://doi.org/10.1007/BF00021542
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00021542