Abstract
We have isolated and characterized a full-length cDNA clone (LHCI-15) which specifies a new chlorophyll-binding protein. This protein is associated with the light-harvesting complex of photosystem I (LHCI). The DNA sequence predicts a precursor protein of 270 amino acids, which shares significant homology with the amino acid sequence of another chlorophyll-binding protein; the chlorophyll a/b-binding (Cab) protein of the photosystem II light-harvesting complex (LHCII). There are two extensive regions of homology (at least 45 residues each) which have approximately 50% amino acid sequence identity. These regions coincide with two of the proposed membrane-spanning alpha helices in the Cab proteins of the LHCII and probably include conserved chlorophyll-binding sites. The LHCI-15 cDNA hybridizes to at least 7 genomic EcoRI DNA fragments, which are very closely related at the nucleotide sequence level.
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Stayton, M.M., Brosio, P. & Dunsmuir, P. Characterization of a full-length petunia cDNA encoding a polypeptide of the light-harvesting complex associated with photosystem I. Plant Mol Biol 10, 127–137 (1987). https://doi.org/10.1007/BF00016150
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DOI: https://doi.org/10.1007/BF00016150