Abstract
Iron chlorosis induced by Fe-deficiency is a widespread nutritional disorder in many woody plants and in particular in grapevine. This phenomenon results from different environmental, nutritional and varietal factors. Strategy I plants respond to Fe-deficiency by inducing physiological and biochemical modifications in order to increase Fe uptake. Among these, acidification of the rhizosphere, membrane redox activities and synthesis of organic acids are greatly enhanced during Fe-deficiency. Grapevine is a strategy I plant but the knowledge on the physiological and biochemical responses to this iron stress deficiency in this plant is still very poor.
In this work four different genotypes of grapevine were assayed for these parameters. It was found that there is a good correlation between genotypes which are known to be chlorosis-resistant and increase in both rhizosphere acidification and FeIII reductase activity. In particular, when grown in the absence of iron, Vitis berlandieri and Vitis vinifera cv Cabernet sauvignon and cv Pinot blanc show a higher capacity to acidify the culture medium (pH was decreased by 2 units), a higher concentration of organic acids, a higher resting transmembrane electrical potential and a greater capacity to reduce FeIII-chelates. On the contrary, Vitis riparia, well known for its susceptibility to iron chlorosis, fails to decrease the pH of the medium and shows a lower concentration in organic acids, lower capacity to reduce FeIII and no difference in the resting transmembrane electrical potential. H Marschner Section editor
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bavaresco, L, Fregoni, M and Fraschini, P 1991 Investigation in iron uptake and reduction by excised roots of different grapevine root-stocks and a V. vinifera cultivar. Plant and Soil 130, 109–113.
Bienfait, H F 1985 Regulated redox processes of the plasmalemma of plant root cells and their function in iron uptake. J. Bioenerg. Biomembr. 17, 73–83.
Bienfait, H F 1988 Mechanisms in Fe-efficiency reactions of higher plants. J. Plant Nutr. 11, 605–629.
Bienfait, H F, Bino, R J, van der Bliek, A M, Duivenvoorden, J F and Fontaine, J M 1983 Characterization of ferric reducing activity in roots of Fe-deficient Phaseulus vulgaris. Physiol. Plant. 59, 196–202.
Brüggeman, W, Moog, P R, Nakagawa, H, Janiesch, P and Kuiper, J C 1990 Plasma membrane-bound NADH:Fe3+-EDTA reductase and iron deficiency in tomato (Lycopersicon esculentum). Is there a turbo reductase? Physiol. Plant. 79, 339–346.
Buckhout, T J, Bell, P F, Luster, D G and Chaney, R L 1989 Iron-stress induced redox activity in tomato (Lycopersicon esculentum Mill.) is localized on the plasma membrane. Plant. Physiol. 90, 151–156.
Chaney, R L, Brown, J C and Tiffin, L O 1972 Obligatory reduction of ferric chelates in iron uptake by soybeans. Plant Physiol. 50, 208–213.
Cocucci, M, Marrè, E, Ballarin-Denti, A and Scacchi, A 1976 Characteristics of fusicoccin-induced changes in transmembrane potential and ion uptake in maize root segments. Plant Sci. Lett. 6, 143–156.
Davies, D D 1973 Control of and by pH. Symp. Soc. Exp. Biol. 27, 513–524.
De Vos, C R, Lubberding, H J and Bienfait, H F 1986 Rhizosphere acidification as a response to iron deficiency in bean plants. Plant Physiol. 81, 842–846.
Fregoni M and Bavaresco L 1985 The Italian contribution to grape breeding. Proc. 4th Int. Symposium on Grapevine Breeding, Verona, Italy. pp 1–6.
Landsberg, E C 1981 Organic acid synthesis and release of hydrogen ions in response to Fe-deficiency stress of mono- and dicotyle-donous plant species. J. Plant Nutr. 3, 579–591.
Lindsay, W L and Schwab, A P 1982 The chemistry of iron in soils and its availability to plants. J. Plant Nutr. 5, 821–840.
Maggioni, A 1980 Iron absorption by excised grapevine roots. Vitis 19, 105–112.
Marrè, E 1979 Integration of solute transport in cereals. In Recent Advances in the Biochemistry of the Cereals. Eds. D L Laydman and R G Wyn Jones. pp 3–25. Academic Press, London, UK.
Marschner, H 1978 Relation between iron supply to grapevine and pH pattern in the nutrient solution. Vitis 17, 152–160.
Mengel, K and Geurtzen, G 1986 Iron chlorosis on calcareous soil. Alkaline nutritional condition as the cause for the chlorosis. J. Plant Nutr. 9, 161–173.
Rabotti, G and Zocchi, G 1994 Plasma membrane-bound H+-ATPase and reductase activities in Fe-deficient cucumber roots. Physiol. Plant. 90, 779–785.
Römheld, V 1987 Different strategies for iron acquisition in higher plants. Physiol. Plant. 70, 231–234.
Römheld, V and Marschner, H 1983 Mechanism of iron uptake by peanut plants. Plant Physiol. 71, 949–954.
Römheld, V and Marschner, H 1986 Mobilization of iron in the rhizosphere of different plant species. In Advances in Plant Nutrition. Eds. B Tinker and A Lauchli. Vol 2, pp 155–204. Praeger Publishers, New York.
Römheld, V, Muller, C and Marschner, H 1984 Localization and capacity of proton pumps in roots of intact sunflower plants. Plant Physiol. 76, 603–606.
Tipton, C L and Thowsen, J 1985 FeIII reduction in cell walls of soybean roots. Plant Physiol. 79, 432–435.
Toulon, V, Sentenac, H, Thibaud, J-B, Davidian, J-C, Moulineau, C and Grignon, C 1992 Role of apoplast acidification by the H+ pump. Effect on the sensitivity to pH and CO2 of iron reduction by roots of Brassica napus L. Planta 186, 212–218.
Varanini, Z and Maggioni, A 1982 Iron reduction and uptake by grapevine roots. J. Plant Nutr. 5, 521–529.
Zocchi, G and Cocucci, S 1990 Fe uptake mechanism in Fe-efficient cucumber roots. Plant Physiol. 92, 908–911.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brancadoro, L., Rabotti, G., Scienza, A. et al. Mechanisms of Fe-efficiency in roots of Vitis spp. in response to iron deficiency stress. Plant Soil 171, 229–234 (1995). https://doi.org/10.1007/BF00010276
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00010276