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Insulin and IGF-I receptors and tyrosine kinase activity in carp ovaries: changes with reproductive cycle

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Résumé

Les récepteurs de l'insuline et du facteur I de croissance de type insulinique (IGF-I) ont été partiellement purifiés à partir d'ovaires de carpe, pris à différents moments de leur cycle reproducteur, à l'aide d'agglutinine de germe de blé. Leurs caractéristiques de liaison et leur activité tyrosine kinase ont été étudiées. Des récepteurs spécifiques pour l'insuline et pour l'IGF-I ont été trouvés. Les récepteurs de l'IGF-I présentent un plus fort taux de liaison (23.8 ± 1.5%), et de plus grandes capacité (965 ± 20 fm/mg) et affinité (KD 0.24 ± 0.03nM), que ceux de l'insuline (respectivement: 4.1 ± 1%, 530 ± 85 fm/mg et 0.85 ± 0.1 nM). Les récepteurs de l'insuline et de l'IGF-I possèdent une activité tyrosine kinase qui ne diffère pas de celle trouvée dans le muscle de la même espéce. Les taux de liaison varient avec la saison, les valeurs maximales étant atteintes, aussi bien pour l'insuline que pour l'lGF-I, à la fin de la période précédant la ponte (Juin). Toutefois, alors que la liaison de l'IGF-I est observée à tous les stades, celle de l'insuline décroit en automne et disparait en hiver; ce qui suggère des rôles différents, pour ces deux peptides, dans la physiologie ovarienne.

Abstract

Insulin and insulin-like growth factor I (IGF-I) receptors from carp ovaries were semipurified with wheat germ agglutinin at different moments of the reproductive cycle and their binding characteristics and tyrosine kinase activity were studied. Specific receptors for insulin and IGF-I were found. IGF-I receptors presented higher binding (23.8 ± 1.5%), number of receptors (965 ± 20fm/mg) and affinity (KD 0.24 ± 0.03nM) than those shown for insulin receptors (4.1 ± 1%, 530 ± 85fm/mg and 0.85 ± 0.1nM, respectively). Insulin and IGF-I receptors have a tyrosine kinase activity which is not different from that found in muscle of the same species. Seasonal changes were found in binding, with maximum values for insulin and IGF-I reached at the end of pre-spawning period (June). However, while IGF-I binding was observed in all stages, insulin binding decreased in autumn and disappeared in winter, which suggests a different role for the two peptides in ovarian physiology.

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Authors and Affiliations

  1. Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Spain

    J. Gutiérrez, M. Párrizas, J. L. Maestro, M. A. Maestro & J. Planas

  2. Instituto de Biociéncias, Universidade de São Paulo, Brasil

    N. Carneiro

Authors
  1. J. Gutiérrez
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  2. M. Párrizas
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  3. N. Carneiro
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  4. J. L. Maestro
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  5. M. A. Maestro
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  6. J. Planas
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Gutiérrez, J., Párrizas, M., Carneiro, N. et al. Insulin and IGF-I receptors and tyrosine kinase activity in carp ovaries: changes with reproductive cycle. Fish Physiol Biochem 11, 247–254 (1993). https://doi.org/10.1007/BF00004572

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