Abstract
The entire amino acid coding sequence of the Na+,K+-ATPase β1 isoform was cloned from the gill of the European eel (Anguilla anguilla) by a PCR based method. The amino acid sequence translated from the nucleotide sequence shared 61.4 and 56.2% homology respectively with previously published Na+,K+-ATPase β1 isoform sequences from the clawed toad (Xenopus laevis) and the ray (Torpedo californica) an elasmobranch fish. The size of the Na+,K+-ATPase β1 mRNA transcript in eel tissues was demonstrated to be 2.35 Kb. Detectable levels of Na+,K+-ATPase β1 mRNA were found at some level in all tissues except liver and cardiac muscle. The level of branchial Na+,K+-ATPase β1 mRNA was observed to increase after the adaptation of fresh water eels to normal or double concentration sea water.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References cited
Ando, M. 1981. Effects of ouabain on chloride movements across the saewater eel intestine. J. Comp. Physiol. 145: 73–79.
Baldisserotto, B. and Mimura, O.M. 1994. Ion transport across the isolated intestinal mucosa of Anguilla anguilla (Pisces). Comp. Biochem. Physiol. 108A: 297–302.
Canfield, V.A., Okamoto, C.T., Chow, D., Dorfman, J., Gros, P., Forte, J.G. and Levenson, R. 1990. Cloning of the H,K-ATPase β subunit. Tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase β subunits. J. Biol. Chem. 265: 19878–19884.
Canfield, V.A. and Levenson, R. 1991. Structural organisation and transcription of the mouse gastric H+,K+-ATPase β subunit gene. Proc. Nat. Acad. Sci. U.S.A. 88: 8247–8251.
Casey, J. and Davidson, N. 1977. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucl. Acids Res. 4: 1539–1552.
Cathala, G., Savouret, J.-F., Mendez, B., West, B.L., Karin, M., Martial, J.A. and Baxter, J.D. 1983. Laboratory Methods — A method for isolation of intact translationally active ribonucleic acid. DNA 2: 329–335.
Epstein, F.H., Silva, P. and Kormanik, G. 1980. Role of Na-K-ATPase in chloride cell function. Am. J. Physiol. 238: R246–R250.
Forrest, J.N. Jr., MacKay, W.C., Gallagher, B. and Epstein, F.H. 1973. Plasma cortisol response to saltwater adaptation in the American eel Anguilla rostrata. Am. J. Physiol. 224: 714–717.
Gloor, S., Antonicek, H., Sweadner, K.J., Pagliusi, S., Frank, R., Moos, M. and Schachner, M. 1990. The adhesion molecule on glia (AMOG) is a homologue of the β subunit of the Na,K-ATPase. J. Cell Biol. 110: 165–174.
Good, P.J., Richter, K. and Dawid, I.B. 1990. A nervous system-specific isotype of the β subunit of Na+,K+-ATPase expressed during early development of Xenopus laevis. Proc. Nat. Acad. Sci. U.S.A. 87: 9088–9092.
Ho, S.-M. and Chan, D.K.O. 1980. Branchial ATPaes and ionic transport in the eel, Anguilla japonica — I. Na+,K+-ATPase. Comp. Biochem. Physiol. 66B: 255–260.
Horisberger, J.-D., Lemas, V., Kraehenbuhl, J.-P. and Rossier, B.C. 1991. Structure-function relationship of Na,K-ATPase. Ann. Rev. Physiol. 53: 565–584.
Jacobson, A. 1987. Purification and fractionation of poly (A)+ RNA. Methods in Enzymology 152: 254–261.
Jaisser, F., Canessa, C.M., Horisberger, J.D. and Rossier, B.C. 1992. Primary sequence and functional expression of a novel ouabain resistant Na,K-ATPase. J. Biol. Chem. 267: 16895–16903.
Jaisser, F., Horisberger, J.D. and Rossier, B.C. 1993. Primary sequence and functional expression of a novel β subunit of the P-ATPase gene family. Pflugers Arch 425: 446–452.
Jorgensen, P.L. 1988. Structural basis for coupling of E1-E2 transitions in αβ-units of renal Na,K-ATPase to Na,Ktranslocation. Prog. Clin. Biol. Res. 268A: 16–38.
Kamiya, M. and Utida, S. 1968. Changes in activity of sodium potassium-activated adenosinetriphosphatase in gills during adaptation of the Japanese eel to sea water. Comp. Biochem. Physiol. 26: 675–685.
Lemas, V.M. and Fambrough, D.M. 1993. Sequence analysis of DNA encoding an Avian Na+,K+-ATPase β2-subunit. Biochim. Biophys. Acta 1149: 339–342.
Maetz, J. and Skadhauge, E. 1968. Drinking rates and gill ionic environment in relation to external salinities in the eel. Nature, Lond. 217: 371–373.
Martin-Vasallo, P., Dackowski, W., Emanuel, J.R. and Levenson, R. 1989. Identification of a putative isoform of the Na,K-ATPase β subunit. Primary structure and tissue-specific expression. J. Biol. Chem. 264: 4613–4618.
Mercer, R.W., Schneider, J.W., Savitz, A., Emanuel, J., Benz, E.J. Jr. and Levenson, R. 1986. Rat brain β-chain gene: primary structure, tissue-specific expression and amplification in ouabain-resistant C+ cells. Mol. Cell Biol. 6: 3884–3890.
Noguchi, S., Noda, M., Takahashi, H., Kawakami, K., Ohta, T., Nagano, K., Hirose T., Inayama, S., Kawamura, M. and Numa, S. 1986. Primary structure of the β-subunit of Torpedo californica (Na+ + K+)-ATPase deduced from the cDNA sequence. FEBS Letts. 196: 315–320.
Ohtsuka, E., Matsuki, S., Ikehara, M., Takahashi, Y. and Matsubara, K. 1985. An alternative approach to deoxyoligonucleotides as hybridisation probes by insertion of deoxyinosine at amgibuous codon positions. J. Biol. Chem. 260: 2605–2608.
Reuben, M.A., Lasater, L.S. and Sachs, G. 1990. Characterisation of a β subunit of the gastric H+/K+-transporting ATPase. Proc. Nat. Acad. Sci. U.S.A. 87: 6767–6771.
Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning a Labortory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbour.
Shamraj, O.I., Melvin, D. and Lingrel, J.B. 1991. Expression of Na,K-ATPase isoforms in human heart. Biochem. Biophys. Res. Commun. 179: 1434–1440.
Takyasu, K., Tamkun, M.M., Siegel, N.R. and Fambrough, D.M. 1987. Expression of hybrid (Na+ +K+)-ATPase molecules after transfection of mouse ltk- cells with DNA encoding the β-subunit of an avian brain sodium pump. J. Biol. Chem. 262: 10733–10740.
Taylor, G.R. 1991. Polymerase chain reaction: basic principles and automation. In PCR: A Practical Approach. pp. 1–14. Edited by M.J. McPherson, P. Quirke and G.R. Taylor, Oxford University Press, Oxford.
Thompson, A.J. and Sargent, J.R. 1977. Changes in the levels of chloride cells and (Na++K+)-dependent ATPase in the gills of yellow and silver eels. J. Exp. Zool. 200: 33–40.
Utida, S., Kamiya, M. and Shirai, N. 1971. Relationship between the activity of Na+,K+-activated adenosinetriphosphatase and the number of chloride cells in eel gills with the special reference to seawater adaptation. Comp. Biochem. Physiol. 38A: 443–447.
Verry, F., Kairouz, P., Schaerer, E., Fuentes, P., Geering, K., Rossier, B.C. and Kraehenbuhl, J.P. 1989. Primary sequence of Xenopus laevis Na+,K+-ATPase and its localisation in A6 kidney cells. Am. J. Physiol. 256: F1034–F1043.
Yancey, P.H. and Somero, G.N. 1980. Methylamine osmoregulatory solutes of elasmobranch fishes counteract urea inhibition of enzymes. J. Exp. Zool. 212: 205–213.
Young, R.M. and Lingrel, J.B. 1987. Tissue distribution of mRNAs encoding the α isoforms and β subunit of rat Na+,K+-ATPase. Biochem. Biophys. Res. Commun. 145: 52–58.
Young, R.M., Shull, G.E. and Lingrel, J.B. 1987. Multiple mRNAs from rat kidney and brain encode a single Na+,K+-ATPase β subunit protein. J. Biol. Chem. 262: 4905–4910.
Yu, H., Ishii, T., Pearson, W.R. and Takyasu, K. 1994. Primary structure of avian H+/K+-ATPase β-subunit. Biochim. Biophys. Acta 1190: 189–192.
Author information
Authors and Affiliations
Additional information
To whom correspondence should be addressed.
Rights and permissions
About this article
Cite this article
Cutler, C.P., Sanders, I.L., Hazon, N. et al. Primary sequence, tissue specificity and mRNA expression of the Na+,K+ -ATPase β1 subunit in the European eel (Anguilla anguilla). Fish Physiol Biochem 14, 423–429 (1995). https://doi.org/10.1007/BF00003380
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00003380