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Metabolic enzyme activities in larvae of the African catfish, Clarias gariepinus: changes in relation to age and nutrition

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Abstract

The influence of ontogeny and nutrition on metabolic enzyme activities in larvae of the African catfish, Clarias gariepinus, was studied. After start of exogenous feeding, the larvae were reared for 10 days under three different nutritional conditions: Artemia nauplii, a dry starter diet, and starvation. The live feed gave the best growth (96 mg within 10 days) whereas the dry diet resulted in low growth (33 mg). This growth difference was reflected in larval RNA and DNA concentrations, but not in the levels of soluble protein. Enzymes representing the following aspects of metabolism have been analysed: NADPH generation (G6PDH, ME), glycolysis (PFK, PK), gluconeogenesis (FDPase), amino acid catabolism (GOT, GPT) and oxidative catabolism (CS). All enzymes were present from the start of exogenous feeding onwards, but their maximum specific activities displayed different developmental patterns. In catfish larvae fed on Artemia, G6PDH and ME activities steadily increased with age and weight of the larvae. CS levels remained, after an immediate enhancement upon onset of exogenous feeding, on a rather stable plateau. The amino acid-degrading enzymes GOT and GPT showed maximum levels at days 3–5 of feeding or at a body weight of 10–20 mg, but decreased thereafter. Activities of PFK, PK and FDPase showed low initial levels, and increased significantly with age and size. Based on the ontogenetic patterns of metabolic enzymes, in C. gariepinus larvae an early and a late developmental phase can be distinguished. During the early phase, the glycolytic and gluconeogenetic capacities are low, whereas they are enforced during the later phase. The oxidative capacity is high both during the early and the late phase. The metabolic changes in catfish development coincide with other major ontogenetic events, e.g., alterations of muscle organization, gill morphology, respiration and stomach structure and function. Rearing catfish larvae on a dry diet instead of Artemia partly altered the developmental pattern described: The ontogenetic elevation of CS, PFK and FDPase was delayed and the early peak in GOT and GPT activities was not realized. Particularly during the early developmental phase, the enzyme behaviour of the larvae fed on dry food was similar to that of starved larvae.

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Abbreviations

CS:

citrate synthase

FDPase:

fructose-1,6-diphosphatase

GOT:

glutamate oxaloacetate transaminase

GPT:

glutamate pyruvate transaminase

G6PDH:

glucose-6-phosphate dehydrogenase

ME:

malic enzyme

PFK:

phosphofructokinase

PK:

pyruvate kinase

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  1. Helmut Segner

    Present address: Department of Chemical Ecotoxicology, Centre for Environmental Research, P.O. Box 2, D-04301, Leipzig, Germany

Authors and Affiliations

  1. Department of Zoology, University of Karlsruhe, P.O. Box 6980, D-76128, Karlsruhe, Germany

    Helmut Segner

  2. Department of Fish Culture and Fisheries, Wageningen Agricultural University, P.O. Box 338, 6700, AH, Wageningen, The Netherlands

    Johan Verreth

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Segner, H., Verreth, J. Metabolic enzyme activities in larvae of the African catfish, Clarias gariepinus: changes in relation to age and nutrition. Fish Physiol Biochem 14, 385–398 (1995). https://doi.org/10.1007/BF00003376

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