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Advanced Imaging and Bio Techniques for Convergence Science pp 153–186Cite as

Micro-endoscopy for Live Small Animal Fluorescent Imaging

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1310))

Abstract

Intravital microscopy has emerged as a powerful technique for the fluorescent visualization of cellular- and subcellular-level biological processes in vivo. However, the size of objective lenses used in standard microscopes currently makes it difficult to access internal organs with minimal invasiveness in small animal models, such as mice. Here we describe front- and side-view designs for small-diameter endoscopes based on gradient-index lenses, their construction, their integration into laser scanning confocal microscopy platforms, and their applications for in vivo imaging of fluorescent cells and microvasculature in various organs, including the kidney, bladder, heart, brain, and gastrointestinal tracts, with a focus on the new techniques developed for each imaging application. The combination of novel fluorescence techniques with these powerful imaging methods promises to continue providing novel insights into a variety of diseases.

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Acknowledgments

We thank Mark Myunghwan Choi and Woei-Ming Lee for technical assistance in microscopy; R. Colvin, C. Chase, H. Ploegh, R. Kucherlapati, K. Hung, Thomas Graf, Klaus Ley, and Adam Glick for generous gifts of transgenic mice and mouse models; Dr. Barry Stipp for providing antibodies; and C. Lin, D. Cote, L. Kaplan, M. Ferrari, and G.Y. Koh for discussions which contributed to previous versions of the component manuscripts.

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  1. Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea

    Bjorn Paulson

  2. Department of Convergence Medicine University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Jun Ki Kim

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Editors and Affiliations

  1. Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Jun Ki Kim

  2. Department of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (Republic of)

    Jeong Kon Kim

  3. Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Chan-Gi Pack

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Paulson, B., Kim, J.K. (2021). Micro-endoscopy for Live Small Animal Fluorescent Imaging. In: Kim, J.K., Kim, J.K., Pack, CG. (eds) Advanced Imaging and Bio Techniques for Convergence Science. Advances in Experimental Medicine and Biology, vol 1310. Springer, Singapore. https://doi.org/10.1007/978-981-33-6064-8_8

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