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Glycoconjugate Vaccines Used for Prevention from Biological Agents: Tandem Mass Spectrometric Analysis

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Detection of Chemical, Biological, Radiological and Nuclear Agents for the Prevention of Terrorism

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Abstract

In this review, we present the determination of the various glycation sites of synthetic neoglycoconjugates formed by conjugation of the antigenic saccharide hapten to BSA using tandem mass spectrometry. The ratio of hapten: BSA was determined by the matrix-assisted laser desorption/ionization-TOF/TOF-MS analyses of the glycoconjugates. We also tentatively propose that all glycated residues are located mainly near the outer surface of the protein.

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Authors and Affiliations

  1. Department of Chemistry, Memorial University of Newfoundland, 232 Elizabeth Avenue, A1B 3X7, St. John’s, NL, Canada

    Farid Jahouh & Joseph Banoub

  2. Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL, A1B 3X9, Canada

    Wael L. L. Demian & Robert J. Brown

  3. NIDDK, LBC, National Institutes of Health, Bethesda, MD, 20892-0815, USA

    Rina Sakksena, Shu-jie Hou & Pavol Kováč

  4. Department of Chemistry, University of Quebec at Montreal, CP 8888, Montreal, QC, H3C 3P8, Canada

    René Roy

  5. Fisheries and Oceans Canada, Science Branch, Special Projects, St John’s, NL, A1C 5X1, Canada

    Joseph Banoub

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  1. Farid Jahouh
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    Joseph Banoub

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Jahouh, F. et al. (2014). Glycoconjugate Vaccines Used for Prevention from Biological Agents: Tandem Mass Spectrometric Analysis. In: Banoub, J. (eds) Detection of Chemical, Biological, Radiological and Nuclear Agents for the Prevention of Terrorism. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9238-7_16

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