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Simultaneous Dissolved Oxygen and Redox Measurements: Use of Polarographic, Bioluminescence and Mass Spectrometric Monitoring Combined with Dual-Wavelength Spectrophotometry

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Gas Enzymology

Abstract

Comparison of available methods for O2 determinations reveals that of the few ways of measuring dissolved O2, the polarographic approach as employed in a continuously-stirred reactor open to gases is extremely useful. However it is complemented and extended in terms of versatility and stability by membrane inlet mass spectrometry, and for sensitivity by the bioluminescence method. Combined with dual wavelength spectrophotometry, fluorimetry or chemiluminescence, these experimental systems provide a powerful approach to problems of microbial and cell biochemistry, as they provide continuous readout of direct non-invasive measurements of functions in intact organisms. Applications are presented: studies of O2 effects on microorganisms with an obligate requirement for oxygen (amoeba), a facultative anaerobe (yeast) and aerotolerant and strict anaerobes (rumen and anaerobic digester populations respectively).

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Author information

Authors and Affiliations

  1. Microbiology Department, University College, Newport Road Cardiff, CF2 1TA, Wales, UK

    D. Lloyd

Authors
  1. D. Lloyd
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Editor information

Editors and Affiliations

  1. Institutes of Biochemistry and Chemistry, Odense University, Odense, Denmark

    H. Degn , R. P. Cox  & H. Toftlund ,  & 

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© 1985 D. Reidel Publishing Company

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Lloyd, D. (1985). Simultaneous Dissolved Oxygen and Redox Measurements: Use of Polarographic, Bioluminescence and Mass Spectrometric Monitoring Combined with Dual-Wavelength Spectrophotometry. In: Degn, H., Cox, R.P., Toftlund, H. (eds) Gas Enzymology. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5279-9_3

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  • DOI: https://doi.org/10.1007/978-94-009-5279-9_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8831-2

  • Online ISBN: 978-94-009-5279-9

  • eBook Packages: Springer Book Archive

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