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Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses

  • Conference paper
Book cover Positive-Strand RNA Viruses

Part of the book series: Archives of Virology Supplementum ((ARCHIVES SUPPL,volume 9))

Summary

Crystallographic and molecular biological studies of T = 3 nodaviruses (180 identical subunits in the particle) and T = 4 tetraviruses (240 identical subunits in the particle) have revealed similarity in both the architecture of the particles and the strategy for maturation. The comparative studies provide a novel opportunity to examine an apparent evolution of particle size, from smaller (T = 3) to larger (T = 4), with both particles based on similar subunits. The BBV and FHV nodavirus structures are refined at 2.8 Å and 3 Å respectively, while the NωV structure is at 6 Å resolution. Nevertheless, the detailed comparisons of the noda and tetravirus X-ray electron density maps show that the same type of switching in subunit twofold contacts is used in the T = 3 and T = 4 capsids, although differences must exist between quasi and icosahedral threefold contacts in the T = 4 particle that have not yet been detected. The analyses of primary and tertiary structures of noda and tetraviruses show that NωV subunits undergo a post assembly cleavage like that observed in nodaviruses and that the cleaved 76 C-terminal residues remain associated with the particle.

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Author information

Author notes

  1. L. Liljas

    Present address: Biomedical Center, Department of Molecular Biology, University of Uppsala, Uppsala, Sweden

  2. A. Fisher

    Present address: Institute for Enzyme Research, University of Wisconsin, Madison, Wisconsin, USA

Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA

    Dr. J. E. Johnson, S. Munshi, L. Liljas, D. Agrawal, N. H. Olson, V. Reddy, A. Fisher, B. McKinney, T. Schmidt & T. S. Baker

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  1. Dr. J. E. Johnson
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  2. S. Munshi
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  3. L. Liljas
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  4. D. Agrawal
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  5. N. H. Olson
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  6. V. Reddy
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  7. A. Fisher
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  8. B. McKinney
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  9. T. Schmidt
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  10. T. S. Baker
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Editor information

Editors and Affiliations

  1. Department of Biology, Georgia State University, Atlanta, Georgia, USA

    Margo A. Brinton

  2. Arthropod-borne and Infectious Disease Laboratory (AIDL), Colorado State University, Fort Collins, Colorado, USA

    Charles H. Calisher

  3. Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin, USA

    Roland Rueckert

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© 1994 Springer-Verlag

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Johnson, J.E. et al. (1994). Comparative studies of T = 3 and T = 4 icosahedral RNA insect viruses. In: Brinton, M.A., Calisher, C.H., Rueckert, R. (eds) Positive-Strand RNA Viruses. Archives of Virology Supplementum, vol 9. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9326-6_48

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  • DOI: https://doi.org/10.1007/978-3-7091-9326-6_48

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82522-8

  • Online ISBN: 978-3-7091-9326-6

  • eBook Packages: Springer Book Archive

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