Skip to main content
SpringerLink
Log in
Book cover

Ruminant Pestivirus Infections pp 29–40Cite as

Bovine viral diarrhea virus proteins and their antigenic analyses

  • Conference paper

Part of the book series: Archives of Virology ((ARCHIVES SUPPL,volume 3))

Summary

Bovine Viral Diarrhea Virus (BVDV) polypeptides present in infected cells are the result of the processing of the polyprotein translated from the large single open reading frame of the BVDV genomic RNA. The presence of these proteins in infected cells was studied by radiolabeling under hypertonic conditions and with the aid of radioimmunoprecipitation. The genomic mapping of these polypeptides suggests a complex pattern of processing which involves cellular and viral proteases. The consistent absence of 80k in noncytopathic isolates of BVDV suggests that the processing of the viral polyprotein is different in cytopathic and noncytopathic biotypes of BVDV. The antigenic structure of BVDV was studied with a panel of monoclonal antibodies (MABs) prepared against the Singer isolate of BVDV. Neutralizing MABs were found to bind the 56–58k polypeptide, providing evidence that this glycoprotein is present on the surface of the virion and carries neutralization epitopes. Antigenic analyses with the panel of MABs reveals extensive antigenic heterogeneity among BVDV field isolates. MABs were used to determine the frequency of neutralization escape mutants in stocks of BVDV. Plaque-purified BVDV stocks contain neutralization escape mutants with a frequency of 10-2.47

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Almond JW, Stanway G, Cann AJ, Westrop GD, Evans DM, Ferguson M, Minor PD, Schild GC (1984) New poliovirus vaccines: a molecular approach. Vaccine 2: 177–82

    Article  PubMed  CAS  Google Scholar 

  2. Baker JC (1987) Bovine viral diarrhea virus: a review. J Am Vet Med Assoc 190: 1449–58

    PubMed  CAS  Google Scholar 

  3. Birrer MJ, Udem S, Nathenson S, Bloom BR (1981) Antigenic variants of measles virus. Nature 293: 67

    Article  PubMed  CAS  Google Scholar 

  4. Bolin SR, McClurkin AW, Cuthp RC, Coria MF (1985) Severe cHnical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. Am J Vet Res 46: 573–576

    PubMed  CAS  Google Scholar 

  5. Bolwell C, Parry NR, Rowlands DJ, Brown F, Ouldridge EJ (1986) Foot and mouth disease virus variants with broad and narrow antigenic spectra. In: Lerner RA, Channock RN, Brown F (eds). Vaccines 86. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p 51

    Google Scholar 

  6. Brownlie J, Clarke MC, Howard CJ (1984) Experimental production of fatal mucosal disease in cattle. Vet Ree 114: 535–537

    Article  CAS  Google Scholar 

  7. Collett MS, Anderson DK, Retzel E (1988) Comparisons of the pestivirus bovine viral diarrhoea virus with members of the flaviviridae. J Gen Virol 69: 2637–43

    Article  PubMed  Google Scholar 

  8. Collett MS, Larson R, Beizer SK, Retzel E (1988) Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165: 200–208

    Article  PubMed  CAS  Google Scholar 

  9. Collett MS, Larson R, Gold C, Strick D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the pestivirus Bovine Viral Diarrhea virus. Virology 165: 191–199

    Article  CAS  Google Scholar 

  10. Commerce Dpt, Bureau of the Census (1984) 1982 Census of Agriculture. US Government Publications

    Google Scholar 

  11. Corapi WV, Donis RO, Dubovi EJ (1988) Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. J Virol 62: 2823–2827

    PubMed  CAS  Google Scholar 

  12. Crainic R, Couillin P, Blondel B, Cabau N, Bouse A, Horodniceau F (1983) Natural variation of pohovirus epitopes. Infect Immun 41: 1217–23

    PubMed  CAS  Google Scholar 

  13. Diamond DC, Jameson BA, Bonin J, Kohara M, Abe S, Crainic R, Wimmer E (1985) Antigenic variation and resistance to neutralization in polio virus type 1. Science 229: 1090–93

    Article  PubMed  CAS  Google Scholar 

  14. Done JT, Terlecki S, Richardson C, Harkness JW, Sands JJ, Patterson DSP, Sweasey D, Shaw IG (1980) Bovine virus diarrhea-mucosal disease virus: pathogenicity for the fetal calf following maternal infection. Vet Ree 108: 473–479

    Article  Google Scholar 

  15. Donis RO, Bean WJ, Webster RG (1989) Distinct hneages of influenza virus H4 hemagglutinin genes in different regions of the world. Virology 169: 408–417

    Article  PubMed  CAS  Google Scholar 

  16. Donis RO, Corapi WV, Dubovi EJ (1988) Neutralizing monoclonal antibodies to BVD virus bind to the 56–58k polypeptide. J Gen Virol 69: 77–86

    Article  PubMed  CAS  Google Scholar 

  17. Donis RO, Dubovi EJ (1987) Glycoproteins of bovine viral diarrhea-mucosal disease virus in infected bovine cells. J Gen Virol 68: 1607–1616

    Article  PubMed  CAS  Google Scholar 

  18. Donis RO, Dubovi EJ (1987) Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. Virology 58: 168–173

    Article  Google Scholar 

  19. Donis RO, Dubovi J (1987) Characterization of bovine viral diarrhea-mucosal disease virus-specific proteins in bovine cells. J Gen Virol 68: 1597–1605

    Article  PubMed  CAS  Google Scholar 

  20. Dubovi EJ, White T, Cordell B, Dales B, Donis RO (1987) Expression of a glycoprotein antigen of bovine viral diarrhea virus that eHcits neutralizing antibodies in mice. In: Chanock M, Lerner R (eds) Modern approaches to new vaccines. Cold Spring Harbor Laboratory, New York, pp 435–439

    Google Scholar 

  21. Eigen M (1971) Self-organization of matter and the evolution of biological macro- molecules. Naturwissenschaften 58: 465–482

    Article  PubMed  CAS  Google Scholar 

  22. Eigen M, Schuster P (1979) The hypercycle: a principle of natural self-organization. Springer, Berhn Heidelberg New York Tokyo

    Google Scholar 

  23. Emini EA, Jameson BA, Lewis AJ, Larsen GR, Wimmer E (1982) Pohovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J Virol 43: 997–1002

    PubMed  CAS  Google Scholar 

  24. Gillespie JH, Coggins L, Thompson J, Baker JA (1961) Comparison by neutralization tests of strains of virus isolated from virus diarrhea and mucosal disease. Cornell Vet 51: 155–159

    PubMed  CAS  Google Scholar 

  25. Gillespie JH, Madin SH, Darby NB (1962) Cellular resistance in tissue culture, induced by noncytopathogenic strains, to a cytopathogenic strain of virus diarrhea virus of cattle. Proc Soc Exp Biol Med 110: 248–250

    PubMed  CAS  Google Scholar 

  26. Goudswaard J, Van Der Donk JA, Noordzij A (1978) Protein A reactivity of various mammalian immunoglobulins. Scand J Immunol 8: 21–28

    Article  PubMed  CAS  Google Scholar 

  27. Harkness JW (1987) The control of bovine viral diarrhoea virus infection. Ann Rech Vet 18: 167–74

    PubMed  CAS  Google Scholar 

  28. Harkness JW, Roeder PL, Drew T, Wood L, Jeffrey M (1985) The efficacy of an experimental inactivated BVD-MD vaccine. CEC seminar: Pestivirus infection of ruminants

    Google Scholar 

  29. Kessler SW (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol 115: 1617–1624

    PubMed  CAS  Google Scholar 

  30. Kniazeff AJ, Huck RA, Jarrett WFH, Prichard WR, Ramsey FK, Schipper IA, Stober M, Liess B (1961) Antigenic relationship of some isolates of bovine viral diarrhea-mucosal disease viruses from the United States, Great Britain and West Germany. Vet Ree 73: 768–769

    Google Scholar 

  31. Kniazeff AJ, Pritchard WR (1960) Antigenic relationships in the bovine viral diarrhea- mucosal disease complex. Proceedings U.S.A.H.A. 64: 345–350

    Google Scholar 

  32. Lee KM, Gillespie JH (1957) Propagation of virus diarrhea virus of cattle in tissue cuhure. Am J Vet Res 18: 952–953

    PubMed  CAS  Google Scholar 

  33. Lobmann M, Charher P, Florent G, Zygraich N (1984) Clinical evaluation of a temperature sensitive bovine viral diarrhea vaccine strain. Am J Vet Res 45: 2498–2504

    PubMed  CAS  Google Scholar 

  34. Nuss DL, Oppermann H, Koch G (1975) Selective inhibition of host protein synthesis in RNA-virus-infected cells. Proc Nat Acad Sci 72: 1258–1262

    Article  PubMed  CAS  Google Scholar 

  35. Office for Statistics, European Community ( 1989 ) Agricultural Statistical Yearbook 1989. Brussels-Luxembourg CECA-CEE-CEEA

    Google Scholar 

  36. Palese P, Young JF (1982) Variation of influenza A, B, and C viruses. Science 215: 1468–74

    Article  PubMed  CAS  Google Scholar 

  37. Peters AR (1987) Vaccines for respiratory disease in cattle. Vaccine 5: 164–69

    Article  PubMed  CAS  Google Scholar 

  38. Portner A, Webster RG, Bean WJ (1980) Similar frequencies of antigenic variants in Sendai, vesicular stomatitis and influenza A viruses. Virology 104: 235–41

    Article  PubMed  CAS  Google Scholar 

  39. Prabhakar BS, Haspel MD, McChntock PR, Notkins AL (1982) High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374–77

    Article  PubMed  CAS  Google Scholar 

  40. Prabhakar BS, Menegus MA, Notkins AL (1985) Detection of conserved and non- conserved epitopes on Coxsackie virus B4: frequency of antigenic change. Virology 146: 302–7

    Article  PubMed  CAS  Google Scholar 

  41. Sheshberadaran H, Norrby E (1986) Characterization of epitopes on the measles virus hemagglutinin. Virology 152: 58–67

    Article  PubMed  CAS  Google Scholar 

  42. Svitkin YV, Lyapustin VN, Lashkevich VA, Agol VI (1984) Differences between translation products of tick-borne encephalitis virus RNA in cell-free systems from Krebs-2 cells and rabbit reticulocytes: involvement of membranes in the processing of nascen precursors of flavivirus structural proteins. Virology 135: 536–41

    Article  PubMed  CAS  Google Scholar 

  43. Underdahl NR, Grace OD, Hoerlein AB (1957) Cultivation in tissue culture of cytopathogenic agent from bovine mucosal disease. Proc Soc Exp Biol Med 94: 795–797

    PubMed  CAS  Google Scholar 

  44. Vonderfecht HE (1980) Why I recommend vaccinating cattle against bovine virus diarrhea (BVD). VMS AC Vet Med Small Anim CHn 75: 853–858

    CAS  Google Scholar 

  45. Webster RG, Laver WG, Air GM, Schild GC (1982) Molecular mechanisms of variation in influenza viruses. Nature 296: 115–21

    Article  PubMed  CAS  Google Scholar 

  46. Wellink J, Van-Kammen A (1988) Proteases involved in the processing of viral polyproteins. Brief review. Arch Virol 98: 1–26

    Article  PubMed  CAS  Google Scholar 

  47. Wiktor TJ, Koprowski H (1980) Antigenic variants of rabies virus. J Exp Med 152: 99–16

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. W. V. Corapi

    Present address: Department of Microbiology, Parasitology and Immunology, NYS College of Veterinary Medicine, Ithaca, NY, 14853, USA

Authors and Affiliations

  1. Department of Veterinary Science, University of Nebraska, Lincoln, NE, 68583-0905, USA

    R. O. Donis

  2. Diagnostic Laboratory, NYS College of Veterinary Medicine, Ithaca, New York, USA

    W. V. Corapi & E. J. Dubovi

Authors
  1. R. O. Donis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. V. Corapi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. J. Dubovi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Tierärztliche Hochschule Hannover, Hannover, Federal Republic of Germany

    B. Liess

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag

About this paper

Cite this paper

Donis, R.O., Corapi, W.V., Dubovi, E.J. (1991). Bovine viral diarrhea virus proteins and their antigenic analyses. In: Liess, B., Moennig, V., Pohlenz, J., Trautwein, G. (eds) Ruminant Pestivirus Infections. Archives of Virology, vol 3. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9153-8_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-9153-8_4

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82279-1

  • Online ISBN: 978-3-7091-9153-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation