Abstract
Cardiac dysfunction can occur after intracerebral hemorrhage (ICH). This study examined the expression of heat shock proteins (HSPs) in the heart after ICH in aged rats and whether deferoxamine (DFX), an iron chelator, affects HSP expression. Male Fischer 344 rats (18 months old) received an injection of 100 μl autologous blood into the right caudate, whereas sham-operated rats had a needle insertion. The rats were treated with DFX or vehicle at 2 and 6 h after ICH and then every 12 h for 3 days. Rats were killed 3 days after ICH, and the hearts were sampled for Western blot analysis of HSP-27 and HSP-32. Western blotting showed that levels of HSP-32 were reduced in the heart after ICH (p < 0.05), and this reduction was normalized by DFX (p < 0.05). DFX had no effects on heart HSP-32 levels in sham-operated rats. ICH also resulted in a reduction in HSP-27 (p < 0.05), but DFX treatment reduced HSP-27 further (p < 0.05). In addition, DFX reduced HSP-27 levels in sham rats. In conclusion, ICH causes HSP-27 and -32 reductions in the heart of aged rats. Deferoxamine treatment has different effects on the expression of HSP-27 and HSP-32.
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References
Samuels MA (2007) The brain-heart connection. Circulation 116:77–84
Garrett MC, Komotar RJ, Starke RM, Doshi D, Otten ML, Connolly ES (2009) Elevated troponin levels are predictive of mortality in surgical intracerebral hemorrhage patients. Neurocrit Care 12(2):199–203
Hurst JW (2003) Electrocardiographic changes in intracranial hemorrhage mimicking myocardial infarction. N Engl J Med 349:1874–1875, author reply 1874–1875
Jung F, Setzer M, Hohnloser SH (2001) Severe intracranial bleeding mimicking acute inferior myocardial infarction with right ventricular involvement. Cardiology 95:48–50
Laowattana S, Zeger SL, Lima JA, Goodman SN, Wittstein IS, Oppenheimer SM (2006) Left insular stroke is associated with adverse cardiac outcome. Neurology 66:477–483
Fang CX, Wu S, Ren J (2006) Intracerebral hemorrhage elicits aberration in cardiomyocyte contractile function and intracellular Ca2+ transients. Stroke 37:1875–1882
Min J, Farooq MU, Greenberg E, Aloka F, Bhatt A, Kassab M, Morgan JP, Majid A (2009) Cardiac dysfunction after left permanent cerebral focal ischemia: the brain and heart connection. Stroke 40:2560–2563
Gong Y, Hua Y, Keep RF, Hoff JT, Xi G (2004) Intracerebral hemorrhage: effects of aging on brain edema and neurological deficits. Stroke 35:2571–2575
Xi G, Keep RF, Hoff JT (2006) Mechanisms of brain injury after intracerebral hemorrhage. Lancet Neurol 5:53–63
Gu Y, Hua Y, Keep RF, Morgenstern LB, Xi G (2009) Deferoxamine reduces intracerebral hematoma-induced iron accumulation and neuronal death in piglets. Stroke 40:2241–2243
Okauchi M, Hua Y, Keep RF, Morgenstern LB, Schallert T, Xi G (2010) Deferoxamine treatment for intracerebral hemorrhage in aged rats: therapeutic time window and optimal duration. Stroke 41:375–382
He Y, Hua Y, Lee J, Liu W, Keep RF, Wang MM, Xi G (2010) Brain alpha – and beta-globin expression after intracerebral hemorrhage. Transl Stroke Res 1:48–56
Burger D, Xiang F, Hammoud L, Lu X, Feng Q (2009) Role of heme oxygenase-1 in the cardioprotective effects of erythropoietin during myocardial ischemia and reperfusion. Am J Physiol Heart Circ Physiol 296:H84–H93
Latchman DS (2001) Heat shock proteins and cardiac protection. Cardiovasc Res 51:637–646
Bergeron M, Gidday JM, Yu AY, Semenza GL, Ferriero DM, Sharp FR (2000) Role of hypoxia-inducible factor-1 in hypoxia-induced ischemic tolerance in neonatal rat brain. Ann Neurol 48:285–296
Efthymiou CA, Mocanu MM, de Belleroche J, Wells DJ, Latchmann DS, Yellon DM (2004) Heat shock protein 27 protects the heart against myocardial infarction. Basic Res Cardiol 99:392–394
Acknowledgment
This study was supported by grants NS-017760, NS-039866, NS-052510, and NS-057539 from the National Institutes of Health (NIH) and 0840016N from the American Heart Association (AHA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and AHA.
Conflict of interest statement We declare that we have no conflict of interest.
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Hu, H., Wang, L., Okauchi, M., Keep, R.F., Xi, G., Hua, Y. (2011). Deferoxamine Affects Heat Shock Protein Expression in Heart after Intracerebral Hemorrhage in Aged Rats. In: Zhang, J., Colohan, A. (eds) Intracerebral Hemorrhage Research. Acta Neurochirurgica Supplementum, vol 111. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0693-8_33
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DOI: https://doi.org/10.1007/978-3-7091-0693-8_33
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