Overview
- Nominated as a distinguished doctoral dissertation by the Chinese Academy of Sciences
- Studies the calcium regulation mechanism of T cell receptor (TCR) activation by combining biophysical (mainly nuclear magnetic resonance (NMR)) and biochemical methods
- Optimizes the Nanodisc assembly protocol, improving its compatibility with more membrane proteins
- Couples the NMR study to the analysis of signaling phenomena in living cells
- Recognized as a research highlight by Nature Asia
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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This thesis describes the use of biophysical and biochemical methods to prove that calcium has a positive feedback effect on amplifying and sustaining CD3 phosphorylation and should enhance T-cell sensitivity to foreign antigens. The study presented shows that calcium can regulate the signal pathway in cells not only as a secondary messenger but also through direct interactions with the phospholipid bilayer. The approach used in the thesis also represents an important advance, as it couples the use of nuclear magnetic resonance (NMR) to the analysis of signaling phenomena in living cells. Moreover, the thesis optimizes the Nanodisc assembly protocol, which can broaden its range of applications in membrane protein studies. A preliminary study on the structure of dengue virus NS2B-NS3p in complex with aprotinin, which may help to develop new drugs against the dengue virus, is also included.
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Table of contents (4 chapters)
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Bibliographic Information
Book Title: Study of the Calcium Regulation Mechanism of TCR Activation Using Nanodisc and NMR Technologies
Authors: Yunchen Bi
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-3-662-54618-5
Publisher: Springer Berlin, Heidelberg
eBook Packages: Engineering, Engineering (R0)
Copyright Information: Springer-Verlag GmbH Germany 2018
Hardcover ISBN: 978-3-662-54616-1Published: 03 July 2017
Softcover ISBN: 978-3-662-57206-1Published: 12 May 2018
eBook ISBN: 978-3-662-54618-5Published: 20 June 2017
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XII, 79
Number of Illustrations: 41 b/w illustrations
Topics: Biomedical Engineering and Bioengineering, Biological and Medical Physics, Biophysics, Membrane Biology, Immunology, Atomic/Molecular Structure and Spectra