Abstract
Hematopoietic growth factor receptors, in general, are integral membrane proteins composed of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular signal-transducing domain. Soluble receptors that lack the transmembrane domain have been shown to be potent immunomodulators of their respective ligands [1–6]. Since IL-6 has been shown to be a central growth factor for myeloma cells [7], an sIL-6R may modulate myeloma cell growth. A novel IL-6R mRNA was isolated from human myeloma cells that exhibits a deletion of the entire transmembrane domain [8]. The corresponding cDNA was sequenced, expressed in fibroblasts, and the functional activity of the protein product was determined using an IL-6 dependent cell line.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fanslow WC, Sims JE, Sasenfeld H, Morrissey PJ, Gillis S, Dower SK, Widmer MB (1990) Regulation of alloreactivity in vivo by a soluble form of the interleukin-1 receptor. Science 248: 739 - 742.
Weisman HF, Bartow T, Leppo MK, Marsh HC, Carson GR, Concino MF, Boyle MP, Roux KH, Weisfeldt ML, Fearon DT (1990) Soluble human complement receptor type 1: in vivo inhibitor of complement suppressing post-ischemic myocardial inflammation and necrosis. Science 249: 146 - 151.
Ward RHR, Capon DJ, Jett CM, Murthy KK, Mordenti J, Lucas C, Frie SW, Prince AM, Green JD, Eichberg JW (1991) Prevention of HIV-1 IIIB infection in chimpanzees by CD4 immunoadhesion. Nature 352: 434–436.
Nemerow GR, Mullen JJ, Dickson PW, Cooper NR (1990) Soluble recombinant CR2 (CD21) inhibits Epstein-Barr virus infection. J Virol 64: 1348–1352.
Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber W, Merluzzi VJ (1990) A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Nature 344: 70–72.
Fanslow WC, Clifford KN, Park LS, Rubin AS, Voice RF, Beckmann MP, Widmer MB (1991) Regulation of alloreactivity in vivo by IL-4 and the soluble IL-4 receptor. J of Immunol 147: 535–540.
Kishimoto T (1989) The biology of Interleukin-6, Blood 74: 1–10.
Lust JA, Donovan KA, Kline MP, Greipp PR, Kyle RA, Maihle NJ (1992) Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor. Cytokine 4 (2): 96–100.
Jelinek DF, Ahmann GJ, Greipp PR, Jalal SM, Westendorf JJ, Katzmann JA, Kyle RA, Lust JA (1993) Coexistence of aneuploid subclones within a myeloma cell line that exhibits clonal immunoglobulin gene rearrangement: clinical implications. Cancer Research 53: 5320–5327.
Yamasaki K, Taga T, Hirata Y, Yawata H, Kawanishi Y, Seed B, Taniguchi T, Hirano T, Kishimoto T (1988) Cloning and expression of the human interleukin-6 (BSF-2/IFN0 2) Receptor. Science 241:825–828.
Fukunaga R, Seto Y, Mizushima S, Nagata S. (1990) Three different mRNAs encoding human granulocyte colony-stimulating factor receptor. Proc Natl Acad Sci USA 87, 8702–8706.
Takaki S, Tominaga A, Hitoshi Y, Mita S, Sonoda E, Yamaguchi N, Takatsu K (1990) Molecular cloning and expression of the murine interleukin-5 receptor. EMBO J 9: 4367–4374.
Goodwin RG, Friend D, Ziegler SF, Jerzy R, Falk BA, Gimpel S, Cossman D, Dower SK, March CJ, Namen AE, Park LIS (1990) Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell 60: 941–951.
Raines MA, Liu L, Quan SG, Joe V, DiPersio JF, Golde DW (1991) Identification and molecular cloning of a soluble human granulocyte-macrophage colony-stimulating factor receptor. Proc Natl Acad Sci USA 88: 8203–8207.
Novick D, Engelmann H, Wallach D, Rubinstein M: Soluble cytokine receptors are present in normal human urine. J Exp Med 170: 1409–1414, 1989.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Lust, J.A. et al. (1995). Sequence, Expression and Function of an mRNA Encoding a Soluble Form of the Human Interleukin-6 Receptor (sIL-6R). In: Potter, M., Melchers, F. (eds) Mechanisms in B-Cell Neoplasia 1994. Current Topics in Microbiology and Immunology, vol 194. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79275-5_24
Download citation
DOI: https://doi.org/10.1007/978-3-642-79275-5_24
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-79277-9
Online ISBN: 978-3-642-79275-5
eBook Packages: Springer Book Archive