Abstract
Our laboratory has emphasized in situ hybridization as a means of investigating the expression and distribution of specific mRNA molecules within single cells, particularly in developing chicken muscle cells in culture. Using an in situ hybridization protocol optimized for maintaining the structural integrity of the cell and the native configuration of RNA (Lawrence and Singer et al. 1986b), we investigated the intracellular distribution of specific mRNAs within intact myoblasts and fibroblasts and provided evidence indicating that mRNAs for certain cytoskeletal proteins are localized within specific regions of the cell (Lawrence and Singer 1986).
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© 1989 Springer-Verlag Berlin · Heidelberg
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Singer, R.H., Lawrence, J.B., Silva, F., Langevin, G.L., Pomeroy, M., Billings-Gagliardi, S. (1989). Strategies for Ultrastructural Visualization of Biotinated Probes Hybridized to Messenger RNA In Situ. In: Haase, A.T., Oldstone, M.B.A. (eds) In Situ Hybridization. Current Topics in Microbiology and Immunology, vol 143. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74425-9_5
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DOI: https://doi.org/10.1007/978-3-642-74425-9_5
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