Abstract
RPL15 is a component of the large ribosomal subunit 60S. The sequences of cDNA and DNA of RPL15 were cloned successfully from Arachis hypogaea cultivar Ri Hua 1. These two sequences were analyzed preliminarily. The full length RPL15 cDNA of A. hypogaea contains a 615-bp open reading frame (ORF) which encodes 204 amino acids. The length of RPL15 gene was 1383 bp with three exons and two introns. Primary structure analysis revealed that the molecular weight of the putative RPL15 protein is 24211.09 Da with a theoretical pI of 11.44. The deduced amino acid sequence of RPL15 (A. hypogaea cultivar Ri Hua 1) shared homology with other reported species. The expression of RPL15 transcript showed that RPL15 mRNA was expressed mainly in root. The cDNA of RPL15 was cloned successfully from A. hypogaea in this study. It provides scientific material for enriching and improving the RPL15 gene database.
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Wang H, Zhao LN, Li KZ et al (2006) Overexpression of ribosomal protein L15 is associated with cell proliferation in gastric cancer. BMC Cancer 6:91–98
Yang F, Liu WP (2005) Study on the relation of ribosomal protein gene and human diseases. Chin J Clin Exp Pathol 3:354–356
Lee HS, Mun JH, Kim SG (1999) Characterization of cDNA encoding cytoplasmic ribosomal protein L15 and L27a in petunia (Petunia hybrida): Primary structures and coordinate expression. Gene 2:155–163
Wang Q, Yang C, Zhou J et al (2001) Cloning and characterization of full-length human ribosomal protein L15 cDNA which was overexpressed in esophageal cancer. Gene 263:205–209
Tom M, Waterman MR, Bocking D et al (1999) Cloning and characterization of the L15 ribosomal protein gene homologue from the crayfish Orconectes limosus. Biochem Biophy Res Comm 2:313–316
Xiang Z, Zhang JN, Song P et al (2006) The study of ribosomal protein L15 cDNA sequences as a molecular marker in the teleostei phylogenetic analysis. Hereditas (BEIJING) 28:171–178
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Thompson JD, Gibson TJ, Plewniak F et al (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Tamura K, Dudley J, Nei M et al (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C (T)) method. Methods 25:402–408
Acknowledgments
The authors wish to express their sincere thanks to all the laboratory members for continuous technical advice and helpful discussion. This research was supported from Qingdao Science and Technology Support Program (12-1-4-11-(1)-jch, 11-2-4-9-(3)-jch), and China Agricultural Research System (CARS-14).
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Wu, Q. et al. (2014). Molecular Characterization and Expression of Ribosomal Protein L15 Gene (RPL15) From Arachis hypogaea . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_125
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DOI: https://doi.org/10.1007/978-3-642-37922-2_125
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