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Detection of Alternatively Spliced or Processed RNAs in Cancer Using Oligonucleotide Microarray

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RNA and Cancer

Part of the book series: Cancer Treatment and Research ((CTAR,volume 158))

Abstract

Deregulation of gene expression plays a pivotal role in tumorigenesis, so the ability to detect RNA alterations is of great value in cancer diagnosis and management. DNA microarrays have been used to measure changes in mRNA or microRNA level, but less often the change of RNA isoforms. Here we appraise the utilization of microarray in detecting alternatively processed RNAs, which have alternative splice forms, retained introns, or altered 3′ untranslated regions. We cover the methodology and focus on cancer studies. Recent development in parallel or deep sequencing used in transcriptome analysis is also discussed.

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Authors and Affiliations

  1. Department of Molecular Biology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010-3000, USA

    Marieta Gencheva, Lixin Yang, Gong-Biao Lin & Ren-Jang Lin

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  1. Marieta Gencheva
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  2. Lixin Yang
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  3. Gong-Biao Lin
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  4. Ren-Jang Lin
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Correspondence to Ren-Jang Lin .

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Editors and Affiliations

  1. Dept. of Neurology, Robert H. Lurie Cancer Center Center for Genetic Medicine, Chicago, Illinois, USA

    Jane Y. Wu

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Gencheva, M., Yang, L., Lin, GB., Lin, RJ. (2013). Detection of Alternatively Spliced or Processed RNAs in Cancer Using Oligonucleotide Microarray. In: Wu, J. (eds) RNA and Cancer. Cancer Treatment and Research, vol 158. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-31659-3_2

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  • DOI: https://doi.org/10.1007/978-3-642-31659-3_2

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