Abstract
Protein S-nitrosylation, a reverse post translational modification (PTM) of cysteine, has been considered as biomarkers in many diseases. Microfluidic has been widely used for development of novel tools for separation of protein mixture. In this report, we have demonstrated a twodimensional micro-electrophoresis (2D μ-CE) separation of nitrosylated proteins in human colon epithelial adenocarcinoma cells (HT-29). Sodium dodecyl sulphate micro-capillary gel electrophoresis (SDS μ-CGE) and microemulsion electrokinetic chromatography (MEEKC) were used for the first and second dimension of separation electrophoresis, respectively. The effective separation lengths for both dimensions were 10 mm, and electrokinetic injection and separation were used with field strengths at 400 V/cm. To our knowledge, this is the first report on 2D profiling of nitrosylated proteins in the biological samples.
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© 2013 Springer-Verlag Berlin Heidelberg
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Wang, S., Njoroge, S.K., Battle, K., Soper, S.A., Feng, J. (2013). Two-Dimensional Nitrosylated Protein Separation in Poly (methyl methacrylate) Microchips. In: Long, M. (eds) World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China. IFMBE Proceedings, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29305-4_79
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DOI: https://doi.org/10.1007/978-3-642-29305-4_79
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-29304-7
Online ISBN: 978-3-642-29305-4
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