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Imaging in Immunology Research

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Small Animal Imaging

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Abstract

The immune system is central to health and tools to measure its dynamic function are critically needed in research and clinical settings. Molecular imaging modalities such as optical imaging, positron emission tomography (PET), computed tomography, and magnetic resonance imaging enable noninvasive longitudinal studies of immune function throughout the body. In vivo molecular imaging studies in small animals have revealed patterns of immune cell localization, trafficking, and function that cannot be obtained using conventional immune monitoring methods. This chapter reviews applications of optical imaging and PET in the study of immune trafficking and function, models of disease, and cancer immunotherapies with an assessment of the challenges facing the field.

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Acknowledgments

We acknowledge all colleagues who contributed to the work presented here. We acknowledge Andrew Tran for helping with figure preparation and Barbara Anderson for helping with manuscript preparation. JTL is supported by the In Vivo Cellular and Molecular Imaging Centers (ICMIC) Developmental Project Award NIH 5 P50 CA86306. ENG is supported by US National Institutes of Health T32 GM08042 UCLA Medical Scientist Training Program and Interdisciplinary Training in Virology and Gene Therapy T32 A1065067. CGR is supported by National Cancer Institute Grant No. 5U54 CA119347, ICMIC Developmental Project Award NIH P50 CA86306, the CIRM Tools and Technology Grant and the Dana Foundation. ONW is an Investigator of the Howard Hughes Medical Institute.

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Authors and Affiliations

  1. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Jason T. Lee

  2. Crump Institute for Molecular Imaging, Los Angeles, CA, USA

    Evan D. Nair-Gill

  3. Department of Experimental Diagnostic Imaging, University of Texas, MD Anderson Cancer Center, Houston, TX, USA

    Brian A. Rabinovich

  4. Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Caius G. Radu

  5. Howard Hughes Medical Institute, University of California, Los Angeles, CA, 90095-1662, USA

    Owen N. Witte

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  1. Jason T. Lee
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  4. Caius G. Radu
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  5. Owen N. Witte
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Correspondence to Owen N. Witte .

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Editors and Affiliations

  1. Chair of Experimental Molecular Imaging, University of Aachen (RWTH), Pauwelsstraße 20, Aachen, 52074, Germany

    Fabian Kiessling

  2. Laboratory for Preclinical Imaging and Imaging Technology of the Werner Siemens-Foundation, Department of Radiology, Eberhard Karls University of Tübingen, Röntgenweg 13, Tübingen, 72076, Germany

    Bernd J. Pichler

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Lee, J.T., Nair-Gill, E.D., Rabinovich, B.A., Radu, C.G., Witte, O.N. (2011). Imaging in Immunology Research. In: Kiessling, F., Pichler, B. (eds) Small Animal Imaging. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-12945-2_36

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