Abstract
This paper describes a digital system designed for the automatic detection and measurement of the velocity of moving objects in images acquired by means of a common TV-camera mounted onto a microscope. The main characteristics of this system are the following: 1) it can perform a real-time gray level difference between two successive frames in order to detect moving objects and to suppress stationary objects (subtraction procedure); usually the delay between two successive frames varies linearly from 40 msec to 1920 msec; 2) it reduces the size of images resulting from the subtraction procedure (difference images) and stores them in the frame memory; the result of these operation, all performed in real-time, is a film of time sequences; 3) it performs an automatic labelization in order to recognize the moving microorganisms and to calculate their area in each difference image; 4) it calculates and plots the variation of the average area of the cells moving in the microscope field; 5) it completes the analysis in few seconds.
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Coltelli, P., Evangelisti, M., Evangelista, V., Gualtieri, P. (2007). Real-Time Measurement and Analysis of Translational and Rotational Speeds of Moving Objects in Microscope Fields. In: Perner, P., Salvetti, O. (eds) Advances in Mass Data Analysis of Signals and Images in Medicine, Biotechnology and Chemistry. MDA 2007. Lecture Notes in Computer Science(), vol 4826. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-76300-0_13
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DOI: https://doi.org/10.1007/978-3-540-76300-0_13
Publisher Name: Springer, Berlin, Heidelberg
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