Abstract
This article reviews initial results from an experimental system designed to identify molecules which regulate gene activity. In particular, the experiments described are concerned with the transcription of cloned DNA’s as studied by microinjection into Xenopus oocytes. We first discuss the rationale behind this experimental approach and then summarize evidence which demonstrates that cloned tRNA genes direct the synthesis of genuine tRNA’s when injected into oocytes. Other experiments described focus on the idea that oocyte injections can be used to identify regions of DNA which contain the information necessary for the accurate transcription of genes. Finally, the prospects for reisolating injected DNA’s from oocytes to look for hypothetical controlling molecules are discussed.
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Melton, D.A., Cortese, R., de Robertis, E.M., Trendelenburg, M.F., Gurdon, J.B. (1980). Gene Injections into Amphibian Oocytes. In: McKinnell, R.G., DiBerardino, M.A., Blumenfeld, M., Bergad, R.D. (eds) Differentiation and Neoplasia. Results and Problems in Cell Differentiation, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-38267-6_2
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DOI: https://doi.org/10.1007/978-3-540-38267-6_2
Publisher Name: Springer, Berlin, Heidelberg
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