Abstract
The study of the kinetics of DNA renaturation has yielded much information about the organization of the DNA in eukaryotes into families of nucleotide sequences (Britten and Kohne, 1967, 1968; Wetmur and Davidson, 1968). With a few exceptions the entire assemblage of families of related base sequences of any one organism is treated as a single body of DNA. It is possible to study the renaturation behaviour of a family of genes coding for a specific RNA of genes within the total genomic DNA, but such experiments are tedious to carry out and so fax have required a combination of hydroxylapatite fractionation of the renaturing DNA (Flamm et al., 1969) and subsequent identification of the specific DNA segments by RNA/DNA hybridization. By this indirect approach it has been shown, for example, that the 800-fold redundant ribosomal RNA genes of Xenopus laevis form a family of closely related base sequence (Grunstein, unpublished) Similarly, after preselection of ribosomal DNA complements from bacteria by RNA/DNA hybridization the renaturation kinetics for these DNA segments indicate that bacterial ribosomal cistrons are similar in base sequence (Kohne, 1968).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Birnstiel, M. L., Speirs, J., Purdom, I., Loening, U. E.: Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature (Lond.) 219, 454–463 (1968).
Birnstiel, M. L., Grunstein,M., Speirs, J., Hennig,W.: Family of ribosomal genes of Xenopus laevis. Nature (Lond.) 223, 1265–1267 (1969).
Birnstiel, M. L., Sells,B., Purdom,I.: Kinetik complexity of RNA molecules. J. Mol. Biol. 63, 21–39(1972).
Bishop, J.O.: The effect of genetic complexity on the timecourse of ribonucleic acid-deoxyribonucleic acid hybridization. Biochem. J. 113, 805–811 (1969).
Bishop, J.O., Robertson, F. W., Burns, J., Melli, M. L.: Methods for the analysis of deoxyribonucleic acid-ribonucleic acid hybridization data. Biochem. J. 115, 361–370 (1969).
Britten, R. J., Kohne, D. E.: Nucleotide sequence repetition in DNA; Carnegie Inst. Year Book 65, 78–106 (1967). Repeated Sequence in DNA; Science 161, 529–540 (1968).
Brown, D. D., Weber, C. S.: Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4s RNA, 5s RNA and ribosomal RNA. J. molec. Biol. 34, 661–660 (1968).
Brownlee, G. G., Sanger, F., Barrel, B. G.: The sequence of 5 s ribosomal ribonucleic acid. J. molec. Biol. 34, 379–412 (1968).
Flamm, W. G., Walker, P.M. B., Mccallum, M.: Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus). J. molec. Biol. 40, 433–443 (1969).
Gillespie,D., Spiegelman,S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membranes. J. molec. Biol. 12, 829–842 (1965).
Kahn, J.: The nucleolar organizer in the mitotic chromosome complement of Xenopus laevis. Quart. J. micr. Sci. 103, 407–409 (1962).
Kohne, D.E.: Isolation and characterization of bacterial ribosomal RNA cristons. Biophys. J. 8, 1104–1118 (1968).
Loening, U. E.: Molecular weights of ribosomal RNA in relation to evolution. J. molec. Biol. 38, 355–365 (1968).
Marmur, J., Ts’o, P.O. P.: Denaturation of deoxyribonucleic acid by formanide. Biochim. biophys. Acta (Amst.) 51, 32–36 (1961).
Mcconaughy, B. L., Laird, C. D., Mccarthy, B. J.: Nucleic acid reassociation in Formamide. Biochemistry 8, 32–89 (1969).
Morrell, P., Smith, I., Dubnan, D., Marmur, J.: Isolation and characterization of low molecular weight ribonucleic acid species from Bacillus subtilis. Biochemistry 6, 258–265 (1967).
Nygaard,A. P., Hall,B.D.: Formation and properties of RNA-DNA complexes. J. molec. Biol. 9, 125–142 (1964).
Quincey, R. V., Wilson, S. H.: The utilization of genes for ribosomal RNA, 5 s RNA, and transfer RNA in liver cells of adult rats. Proc. nat. Acad. Sci. (Wash.) 64, 981–988 (1969).
Rirossa, F., Spiegelman, S.: Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965).
Rirossa, F., Atwood, K. C., Linsley, D. L., Spiegelman, S.: On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA. Natl. Cancer Inst. Monogr. 23, 449–472 (1966).
Speirs, J., Birnstiel, M. L.: in prepatation.
Tartof, K. D., Perry, R. P.: The 5 S genes in D. melanogaster, J. Mol. Biol. 51, 171–183 (1970).
Walker, P. M. B.: The specificity of molecular hybridization in relation to studies on higher organisms, Progress Nuc 1. Acid. Res. Mol. Biol. 9, 301–323 (1969).
Wallace, H., Birnstiel, M. L.: Ribosomal cistrons and the nucleolar organizer. Biochim. biophys. Acta (Amst.) 114, 296–310 (1966).
Wetmur, J. G., Davidson,N.: Kinetics or renaturation of DNA. J. molec. Biol. 31, 349–370 (1968).
Zehavi-Willner, T., Comb, D.: Studies on the relationship between transfer RNA and transfer-like RNA. J. molec. Biol. 16, 250–254 (1966).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1972 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Purdom, I., Williamson, R., Birnstiel, M. (1972). Kinetic Analysis of the Base Sequence Heterogeneity of RNA Molecules by RNA/DNA Hybridization. In: Ursprung, H. (eds) Nucleic Acid Hybridization in the Study of Cell Differentiation. Results and Problems in Cell Differentiation, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-37149-6_3
Download citation
DOI: https://doi.org/10.1007/978-3-540-37149-6_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-22245-4
Online ISBN: 978-3-540-37149-6
eBook Packages: Springer Book Archive