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Arterial Chemoreceptors pp 35–41Cite as

TASK-1 (K2P3) and TASK-3 (K2P9) in Rabbit Carotid Body Glomus Cells

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1071))

Abstract

Glomus cells isolated from rabbit and rat/mouse carotid bodies have been used for many years to study the role of ion channels in hypoxia sensing. Studies show that hypoxia inhibits the inactivating K+ channels (Kv4) in rabbits, but inhibits TASK in rats/mice to elicit the hypoxic response. Because the role of TASK in rabbit glomus cells is not known, we isolated glomus cells from rabbits and studied the expression of TASK mRNA in the whole carotid body (CB), changes in [Ca2+]i and TASK activity. RT-PCR showed that rabbit CB expressed mRNA for TASK-3 and several Kv (Kv2.1, Kv3.1 and Kv3.3). In rabbit glomus cells in which 20 mM KClo elevated [Ca2+], anoxia also elicited a strong rise in [Ca2+]. In cell-attached patches with 140 mM KCl in the pipette, basal openings of ion channels with single-channel conductance levels of 16-pS, 34-pS, and 42-pS were present. TREK-like channels were also observed. In inside-out patches with high [Ca2+]i, BK was activated. The 42-pS channel opened spontaneously and briefly. The 16-pS and 34-pS channels showed properties similar to those of TASK-1 and TASK-3, respectively. TASK activity in cell-attached patches was lower than that in rat glomus cells under identical recording conditions. Hypoxia (~0.5%O2) reduced TASK activity by ~52% and depolarized the cells by ~30 mV. Our results show that the O2-sensitive TASK contributes to the hypoxic response in rabbit glomus cells.

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Acknowledgements

This work was supported by funds from NIH, Rosalind Franklin University, and the Ministry of Science, ICT and Future Planning (NRF-2015R1A5A2008833, Korea).

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Authors and Affiliations

  1. Department of Physiology and Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju, South Korea

    Dawon Kang

  2. Department of Physiology and Biophysics, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA

    Jiaju Wang, James O. Hogan & Donghee Kim

Authors
  1. Dawon Kang
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  2. Jiaju Wang
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  3. James O. Hogan
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  4. Donghee Kim
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Corresponding author

Correspondence to Dawon Kang .

Editor information

Editors and Affiliations

  1. Department of Pediatrics, Johns Hopkins University, Baltimore, MD, USA

    Estelle B. Gauda

  2. Faculty of Medical Sciences, Nova University of Lisbon Faculty of Medical Sciences, Lisbon, Portugal

    Maria Emilia Monteiro

  3. Department of Medicine, University of Chicago , Chicago, IL, USA

    Nanduri Prabhakar

  4. Department of Neuroscience, Cell Biology, Wright State University, Dayton, OH, USA

    Christopher Wyatt

  5. Cellular and Integrative Physiology, University of Nebraska, Omaha, NE, USA

    Harold D. Schultz

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Kang, D., Wang, J., Hogan, J.O., Kim, D. (2018). TASK-1 (K2P3) and TASK-3 (K2P9) in Rabbit Carotid Body Glomus Cells. In: Gauda, E., Monteiro, M., Prabhakar, N., Wyatt, C., Schultz, H. (eds) Arterial Chemoreceptors. Advances in Experimental Medicine and Biology, vol 1071. Springer, Cham. https://doi.org/10.1007/978-3-319-91137-3_4

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