Abstract
The [second generation (2G)] biorefinery is gaining wide attention for the production of biofuels such as bioethanol and coproducts such as xylooligosaccharides using lignocellulosic materials as feedstock. However, enzymatic hydrolysis of pretreated lignocellulosic materials to produce fermentable sugars is a complex-step bioethanol production process. In addition, a bottleneck in lignocellulosic biomass conversion to bioethanol is the cost of these enzymes. Thus, one of the most important objectives and challenges in the production of 2G bioethanol is the development of cost-effective processes at large scale. This chapter gives an overview of enzymatic hydrolysis process, the effect of pretreatment on enzymatic hydrolysis, operational strategies, and reactor design and operation as well as the advances achieved in recent years.
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Abbreviations
- 2G:
-
Second generation
- LCM:
-
Lignocellulosic material
- SHF:
-
Separate hydrolysis and fermentation
- SSF:
-
Simultaneous saccharification and fermentation
- CBP:
-
Consolidated bioprocessing
- SSSF:
-
Semi-simultaneous saccharification and fermentation
- SSCF:
-
Simultaneous saccharification and co-fermentation
- HMF:
-
Hydroxymethylfurfural
- DP:
-
Degree of polymerization
- AFEXTM :
-
Ammonia fiber expansion
- EGW:
-
Eucalyptus globulus wood
- DM:
-
Dry matter
- WIS:
-
Water-insoluble solids
- FPU:
-
Filter paper unit
- STR:
-
Stirred-tank reactor
- RFA:
-
Renewable fuel association
- IEA:
-
International Energy Agency
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Acknowledgements
The authors would like to thank Dr. Heather L. Trajano (Department of Chemical and Biological Engineering, The University of British Columbia, Canada), for her comments and revision of this chapter. Financial support from the Energy Sustainability Fund 2014–05 (CONACYT-SENER), Mexican Centre for Innovation in Bioenergy (Cemie-Bio), Cluster of Bioalcohols (Ref. 249564) is gratefully acknowledged. We also gratefully acknowledge support for this research by the Mexican Science and Technology Council (CONACYT, Mexico) for the infrastructure project—INFR201601 (Ref. 269461) and Basic Science Project-2015-01 (Ref. 254808). The authors Daniela Aguilar and Anely Lara thank to the Mexican Science and Technology Council (CONACYT, Mexico) for their Master fellowship grant, and Elisa Zanuso thanks Energy Sustainability Fund 2014–05 (CONACYT-SENER, Ref. 249564), for Undergraduate fellowship grant.
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Aguilar, D.L. et al. (2018). Operational Strategies for Enzymatic Hydrolysis in a Biorefinery. In: Kumar, S., Sani, R. (eds) Biorefining of Biomass to Biofuels. Biofuel and Biorefinery Technologies, vol 4. Springer, Cham. https://doi.org/10.1007/978-3-319-67678-4_10
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