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3D Bioprinting and 3D Imaging for Stem Cell Engineering

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Bioprinting in Regenerative Medicine

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

Three-dimensional (3D) bio-printing, a technology to create 3D tissue through layer-by-layer approach, offers great capacity to engineer tissue with desired cells, growth factors and biomaterial scaffolds in spatial patterns to mimic the native tissue architecture. With its flexibility and power, the 3D bio-printing technology can also be used to control stem cell fate and creating 3D stem cell niches. Meanwhile, 3D bio-printed tissues often incorporate thick opaque scaffold, dense population of cells, and are often large in size (1–100 mm). Thus, there are significant difficulties in visualizing the biological events within thick tissue constructs using current microscopic techniques. To elucidate the interaction of stem cells with the microenvironment in tissue engineering applications, it is necessary to develop novel molecular imaging techniques to non-invasively observe stem cell fate, cell-cell interactions, and structural features of an engineered tissue in real time. In this chapter, we review the usage of bio-printing technologies in stem cell and tissue engineering application, and the most recent development in the optical molecular imaging techniques for thick tissue imaging.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th Street, 12180, Troy, NY, USA

    Vivian K. Lee Ph.D., Andrew Dias M.S., Mehmet S. Ozturk M.S., Kathleen Chen, Brad Tricomi M.S. Bio., David T. Corr Ph.D., Xavier Intes Ph.D. & Guohao Dai Ph.D.

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  1. Vivian K. Lee Ph.D.
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  1. Sprott Centre for Stem Cell Research, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

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Lee, V. et al. (2015). 3D Bioprinting and 3D Imaging for Stem Cell Engineering. In: Turksen, K. (eds) Bioprinting in Regenerative Medicine. Stem Cell Biology and Regenerative Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-21386-6_2

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