Abstract
Damage from free radicals and reactive oxygen species (ROS) has been linked to cancers. We evaluated the antioxidant potential of taurine. It was tested by using multiple assay systems; reactive oxygen species (ORAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 3-ethyl-benzothiazoline-6-sulfonic acid (ABTS). Taurine exhibited significant antioxidant capacities with 29.97 ± 0.17, 15.15 ± 0.77, 17.51 ± 0.55, 47.97 ± 0.89 mM (trolox equivalent) TE at 1,000 mg/mL. In vitro assay for two types of breast cancer cell deaths were also determined. Cell viability results showed that the percentages of apoptotic human breast cancer cells, MDA-MB 231, after treatment with 25 mM, 50 mM, 100 mM, 250 mM, 500 mM and 1,000 mM taurine for 24 h reached 21.61 %, 22.42 %, 37.70 %, 45.22 %, 67.01 % and 90.62 %, respectively. For the other breast cancer cells, MCF-7, after treatment with 25 mM, 50 mM, 100 mM, 250 mM, 500 mM and 1,000 mM taurine for 24 h, the cell death rates were 14.29 %, 22.38 %, 31.65 %, 42.53 %, 51.73 %, 77.70 %, respectively.
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Abbreviations
- ABTS:
-
3-Ethyl-benzothiazoline-6-sulfonic acid
- DPPH:
-
2,2-Diphenyl-1-picrylhydrazyl
- FRAP:
-
Ferric reducing antioxidant power
- ORAC:
-
Oxygen radical absorbance capacity
- ROS:
-
Reactive oxygen species
- TE:
-
Trolox equivalent
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This work was supported by Konkuk University in 2014.
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Choi, EJ. et al. (2015). Investigation of Antioxidant and Anticancer Potential of Taurine by Means of Multiple Chemical and Biological Assays. In: Marcinkiewicz, J., Schaffer, S. (eds) Taurine 9. Advances in Experimental Medicine and Biology, vol 803. Springer, Cham. https://doi.org/10.1007/978-3-319-15126-7_16
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DOI: https://doi.org/10.1007/978-3-319-15126-7_16
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