Abstract
Persister cells are phenotypic variants of a bacterial population that display tolerance to killing by bactericidal antibiotics. In this chapter we discuss the formation of persisters and their role in biofilm associated infections.
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References
Allison KR, Brynildsen MP, Collins JJ (2011) Metabolite-enabled eradication of bacterial persisters by aminoglycosides. Nature 473:216
Amato SM, Brynildsen MP (2014) Nutrient transitions are a source of persisters in Escherichia coli biofilms. PLoS One 9:e93110
Amato SM et al (2014) The role of metabolism in bacterial persistence. Front Microbiol 5:70
Ammons MC (2010) Anti-biofilm strategies and the need for innovations in wound care. Recent Pat Antiinfect Drug Discov 5:10
Anderl JN, Franklin MJ, Stewart PS (2000) Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrob Agents Chemother 44:1818
Baba T et al (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:2006.0008
Balaban NQ, Merrin J, Chait R, Kowalik L, Leibler S (2004) Bacterial persistence as a phenotypic switch. Science 305:1622
Bernier SP et al (2013) Starvation, together with the SOS response, mediates high biofilm-specific tolerance to the fluoroquinolone ofloxacin. PLoS Genet 9:e1003144
Bigger JW (1944) Treatment of staphylococcal infections with penicillin. Lancet ii:497
Brotz-Oesterhelt H et al (2005) Dysregulation of bacterial proteolytic machinery by a new class of antibiotics. Nat Med 11:1082
Conlon BP et al (2013) Activated ClpP kills persisters and eradicates a chronic biofilm infection. Nature 503:365
Costerton JW et al (1987) Bacterial biofilms in nature and disease. Annu Rev Microbiol 41:435
Darouiche RO et al (1994) Vancomycin penetration into biofilm covering infected prostheses and effect on bacteria. J Infect Dis 170:720
Davis BD (1987) Mechanism of bactericidal action of aminoglycosides. Microbiol Rev 51:341
De Groote VN et al (2009) Novel persistence genes in Pseudomonas aeruginosa identified by high-throughput screening. FEMS Microbiol Lett 297:73
Donegan NP, Cheung AL (2009) Regulation of the mazEF toxin-antitoxin module in Staphylococcus aureus and its impact on sigB expression. J Bacteriol 191:2795
Donegan NP, Thompson ET, Fu Z, Cheung AL (2010) Proteolytic regulation of toxin-antitoxin systems by ClpPC in Staphylococcus aureus. J Bacteriol 192:1416
Dorr T, Vulic M, Lewis K (2010) Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli. PLoS Biol 8:e1000317
Drlica K, Zhao X (1997) DNA gyrase, topoisomerase IV, and the 4-quinolones. Microbiol Mol Biol Rev 61:377
Durfee T, Hansen AM, Zhi H, Blattner FR, Jin DJ (2008) Transcription profiling of the stringent response in Escherichia coli. J Bacteriol 190:1084
Falla TJ, Chopra I (1998) Joint tolerance to beta-lactam and fluoroquinolone antibiotics in Escherichia coli results from overexpression of hipA. Antimicrob Agents Chemother 42:3282
Farrell CM, Grossman AD, Sauer RT (2005) Cytoplasmic degradation of ssrA-tagged proteins. Mol Microbiol 57:1750
Gerdes K, Christensen SK, Lobner-Olesen A (2005) Prokaryotic toxin-antitoxin stress response loci. Nat Rev Microbiol 3:371
Germain E, Castro-Roa D, Zenkin N, Gerdes K (2013) Molecular mechanism of bacterial persistence by HipA. Mol Cell 52:248
Girgis HS, Harris K, Tavazoie S (2012) Large mutational target size for rapid emergence of bacterial persistence. Proc Natl Acad Sci U S A 109:12740
Gristina AG, Hobgood CD, Webb LX, Myrvik QN (1987) Adhesive colonization of biomaterials and antibiotic resistance. Biomaterials 8:423
Hansen S, Lewis K, Vulić M (2008) The role of global regulators and nucleotide metabolism in antibiotic tolerance in Escherichia coli. Antimicrob Agents Chemother 52:2718–2726
Hansen S et al (2012) Regulation of the Escherichia coli HipBA toxin-antitoxin system by proteolysis. PLoS One 7:e39185
Hayes F, Van Melderen L (2011) Toxins-antitoxins: diversity, evolution and function. Crit Rev Biochem Mol Biol 46:386
Helaine S et al (2014) Internalization of Salmonella by macrophages induces formation of nonreplicating persisters. Science 343:204
Jefferson KK, Goldmann DA, Pier GB (2005) Use of confocal microscopy to analyze the rate of vancomycin penetration through Staphylococcus aureus biofilms. Antimicrob Agents Chemother 49:2467
Johnson PJ, Levin BR (2013) Pharmacodynamics, population dynamics, and the evolution of persistence in Staphylococcus aureus. PLoS Genet 9:e1003123
Kaspy I et al (2013) HipA-mediated antibiotic persistence via phosphorylation of the glutamyl-tRNA-synthetase. Nat Commun 4:3001
Keren I, Kaldalu N, Spoering A, Wang Y, Lewis K (2004) Persister cells and tolerance to antimicrobials. FEMS Microbiol Lett 230:13
Keren I, Mulcahy LR, Lewis K (2012) Persister eradication: lessons from the world of natural products. Methods Enzymol 517:387
Kim Y, Wood TK (2010) Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli. Biochem Biophys Res Commun 391:209
Kim JS et al (2011) Selective killing of bacterial persisters by a single chemical compound without affecting normal antibiotic-sensitive cells. Antimicrob Agents Chemother 55:5380
Kirstein J et al (2009) The antibiotic ADEP reprogrammes ClpP, switching it from a regulated to an uncontrolled protease. EMBO Mol Med 1:37
Lafleur MD, Qi Q, Lewis K (2010) Patients with long-term oral carriage harbor high-persister mutants of Candida albicans. Antimicrob Agents Chemother 54:39
Lebeaux D et al. (2014) pH-mediated potentiation of aminoglycosides kills bacterial persisters and eradicates in vivo biofilms. J Infect Dis. doi 10.1093/infdis/jiu286
Leung V, Levesque CM (2012) A stress-inducible quorum-sensing peptide mediates the formation of persister cells with noninherited multidrug tolerance. J Bacteriol 194:2265
Lewis K (2001) Riddle of biofilm resistance. Antimicrob Agents Chemother 45:999
Maisonneuve E, Shakespeare LJ, Jorgensen MG, Gerdes K (2011) Bacterial persistence by RNA endonucleases. Proc Natl Acad Sci U S A 108:13206
Maisonneuve E, Castro-Camargo M, Gerdes K (2013) (p)ppGpp controls bacterial persistence by stochastic induction of toxin-antitoxin activity. Cell 154:1140
Michel A et al (2006) Global regulatory impact of ClpP protease of Staphylococcus aureus on regulons involved in virulence, oxidative stress response, autolysis, and DNA repair. J Bacteriol 188:5783
Moker N, Dean CR, Tao J (2010) Pseudomonas aeruginosa increases formation of multidrug-tolerant persister cells in response to quorum-sensing signaling molecules. J Bacteriol 192:1946
Moyed HS, Bertrand KP (1983) hipA, a newly recognized gene of Escherichia coli K-12 that affects frequency of persistence after inhibition of murein synthesis. J Bacteriol 155:768
Mulcahy LR, Burns JL, Lory S, Lewis K (2010) Emergence of Pseudomonas aeruginosa strains producing high levels of persister cells in patients with cystic fibrosis. J Bacteriol 192:6191
Murakami K et al (2005) Role for rpoS gene of Pseudomonas aeruginosa in antibiotic tolerance. FEMS Microbiol Lett 242:161
Nguyen D et al (2011) Active starvation responses mediate antibiotic tolerance in biofilms and nutrient-limited bacteria. Science 334:982
Norton JP, Mulvey MA (2012) Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli. PLoS Pathog 8:e1002954
Ogura T, Hiraga S (1983) Mini-F plasmid genes that couple host cell division to plasmid proliferation. Proc Natl Acad Sci U S A 80:4784
Spoering AL, Vulic M, Lewis K (2006) GlpD and PlsB participate in persister cell formation in Escherichia coli. J Bacteriol 188:5136
Tipper DJ, Strominger JL (1965) Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine. Proc Natl Acad Sci U S A 54:1133
Tsilibaris V, Maenhaut-Michel G, Van Melderen L (2006) Biological roles of the Lon ATP-dependent protease. Res Microbiol 157:701
Viducic D et al (2006) Functional analysis of spoT, relA and dksA genes on quinolone tolerance in Pseudomonas aeruginosa under nongrowing condition. Microbiol Immunol 50:349
Vrany JD, Stewart PS, Suci PA (1997) Comparison of recalcitrance to ciprofloxacin and levofloxacin exhibited by Pseudomonas aeruginosa biofilms displaying rapid-transport characteristics. Antimicrob Agents Chemother 41:1352
Zhou YN, Jin DJ (1998) The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like “stringent” RNA polymerases in Escherichia coli. Proc Natl Acad Sci U S A 95:2908
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Conlon, B.P., Rowe, S.E., Lewis, K. (2015). Persister Cells in Biofilm Associated Infections. In: Donelli, G. (eds) Biofilm-based Healthcare-associated Infections. Advances in Experimental Medicine and Biology, vol 831. Springer, Cham. https://doi.org/10.1007/978-3-319-09782-4_1
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DOI: https://doi.org/10.1007/978-3-319-09782-4_1
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