Skip to main content
SpringerLink
Log in

High-Resolution Imaging Mass Spectrometry of Human Donor Eye: Photoreceptors Cells and Basal Laminar Deposit of Age-Related Macular Degeneration

  • Conference paper
  • First Online:
Retinal Degenerative Diseases XIX

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1415))

Abstract

Pathologies of the retina are clinically visualized in vivo with OCT and ex vivo with immunohistochemistry. Although both techniques provide valuable information on prognosis and disease state, a comprehensive method for fully elucidating molecular constituents present in locations of interest is desirable. The purpose of this work was to use multimodal imaging technologies to localize the vast number of molecular species observed with matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) in aged and diseased retinal tissues. Herein, MALDI IMS was utilized to observe molecular species that reside in photoreceptor cells and also a basal laminar deposit from two human donor eyes. The molecular species observed to accumulate in these discrete regions can be further identified and studied to attempt to gain a greater understanding of biological processes occurring in debilitating eye diseases such as age-related macular degeneration (AMD).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 349.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 449.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Caprioli RM, Farmer TB, Gile J. Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. Anal Chem. 1997;69(23):4751–60.

    Article  CAS  PubMed  Google Scholar 

  2. Bowrey HE, Anderson DM, Pallitto P, Gutierrez DB, Fan J, Crouch RK, et al. Imaging mass spectrometry of the visual system: advancing the molecular understanding of retina degenerations. Proteomics Clin Appl. 2016;10(4):391–402.

    Article  CAS  PubMed  Google Scholar 

  3. Anderson DMG, Messinger JD, Patterson NH, Rivera ES, Kotnala A, Spraggins JM, et al. Lipid landscape of the human retina and supporting tissues revealed by high resolution imaging mass spectrometry. bioRxiv:2020:2020.04.08.029538.

    Google Scholar 

  4. Zemski Berry KA, Gordon WC, Murphy RC, Bazan NG. Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry. J Lipid Res. 2014;55(3):504–15.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Anderson DM, Spraggins JM, Rose KL, Schey KL. High spatial resolution imaging mass spectrometry of human optic nerve lipids and proteins. J Am Soc Mass Spectrom. 2015;26(6):940–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Stark DT, Anderson DMG, Kwong JMK, Patterson NH, Schey KL, Caprioli RM, et al. Optic nerve regeneration after crush remodels the injury site: molecular insights from imaging mass spectrometryoptic nerve regeneration imaging mass spectrometry. Invest Ophthalmol Vis Sci. 2018;59(1):212–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Grey AC, Schey KL. Age-related changes in the spatial distribution of human lens alpha-crystallin products by MALDI imaging mass spectrometry. Invest Ophthalmol Vis Sci. 2009;50(9):4319–29.

    Article  PubMed  Google Scholar 

  8. Stella DR, Floyd KA, Grey AC, Renfrow MB, Schey KL, Barnes S. Tissue localization and solubilities of alphaA-crystallin and its numerous C-terminal truncation products in pre- and postcataractous ICR/f rat lenses. Invest Ophthalmol Vis Sci. 2010;51(10):5153–61.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Chen Y, Jester JV, Anderson DM, Marchitti SA, Schey KL, Thompson DC, et al. Corneal haze phenotype in Aldh3a1-null mice: in vivo confocal microscopy and tissue imaging mass spectrometry. Chem Biol Interact. 2017;276:9–14.

    Article  CAS  PubMed  Google Scholar 

  10. Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, et al. High resolution MALDI imaging mass spectrometry of retinal tissue lipids. J Am Soc Mass Spectrom. 2014;25(8):1394–403.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Anderson DMG, Ablonczy Z, Koutalos Y, Hanneken AM, Spraggins JM, Calcutt MW, et al. Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas. Sci Rep. 2017;7(1):17352.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Patterson NH, Tuck M, Van de Plas R, Caprioli RM. Advanced registration and analysis of MALDI imaging mass spectrometry measurements through autofluorescence microscopy. Anal Chem. 2018;90(21):12395–403.

    Article  CAS  PubMed  Google Scholar 

  13. Pang CE, Messinger JD, Zanzottera EC, Freund KB, Curcio CA. The onion sign in neovascular age-related macular degeneration represents cholesterol crystals. Ophthalmology. 2015;122(11):2316–26.

    Article  PubMed  Google Scholar 

  14. Hankin J, Barkley R, Murphy R. Sublimation as a method of matrix application for mass spectrometric imaging. J Am Soc Mass Spectrom. 2007;18(9):1646–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Liu A, Chang J, Lin Y, Shen Z, Bernstein PS. Long-chain and very long-chain polyunsaturated fatty acids in ocular aging and age-related macular degeneration. J Lipid Res. 2011;51:3217–29.

    Article  Google Scholar 

  16. Curcio CA, Presley JB, Malek G, Medeiros NE, Avery DV, Kruth HS. Esterified and unesterified cholesterol in drusen and basal deposits of eyes with age-related maculopathy. Exp Eye Res. 2005;81(6):731–41.

    Article  CAS  PubMed  Google Scholar 

  17. Wang L, Li C-M, Rudolf M, Belyaeva OV, Chung BH, Messinger JD, et al. Lipoprotein particles of intraocular origin in human Bruch membrane: an unusual lipid profile. Invest Ophthalmol Vis Sci. 2009;50(2):870–7.

    Article  PubMed  Google Scholar 

  18. Sarks SH. Ageing and degeneration in the macular region: a clinico-pathological study. Br J Ophthalmol. 1976;60(5):324–41.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Tan ACS, Astroz P, Dansingani KK, Slakter JS, Yannuzzi LA, Curcio CA, et al. The plateau, an optical coherence tomographic signature of geographic atrophy: evolution, multimodal imaging, and candidate histology. Invest Ophthalmol Vis Sci. 2017;58(4):2349–58.

    Article  PubMed  PubMed Central  Google Scholar 

  20. Sura AA, Chen L, Messinger JD, Swain TA, McGwin G Jr, Freund KB, et al. Measuring the contributions of basal laminar deposit and Bruch’s membrane in age-related macular degeneration. Invest Ophthalmol Vis Sci. 2020;61(13):19.

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This project was supported by the National Institutes of Health P41 GM103391 (R.M.C.) and R01 EY027948 (C.A.C.). Support was also received by a Research to Prevent Blindness Catalyst Award for Innovative Research Approaches for Age-Related Macular Degeneration to K.L.S.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Vanderbilt University, Nashville, TN, USA

    David M. G. Anderson, Ankita Kotnala, Nathan Heath Patterson, Richard M. Caprioli & Kevin L. Schey

  2. Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN, USA

    David M. G. Anderson, Nathan Heath Patterson, Jeffrey M. Spraggins, Richard M. Caprioli & Kevin L. Schey

  3. Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, AL, USA

    Ankita Kotnala, Jeffrey D. Messinger, Jeffrey M. Spraggins & Christine A. Curcio

  4. Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA

    Jeffrey M. Spraggins

Authors
  1. David M. G. Anderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ankita Kotnala
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeffrey D. Messinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nathan Heath Patterson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jeffrey M. Spraggins
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Christine A. Curcio
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Richard M. Caprioli
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kevin L. Schey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kevin L. Schey .

Editor information

Editors and Affiliations

  1. Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    John D. Ash

  2. Ocular Genomics InstituteDepartment of OphthalmologyMassachusetts Eye and Ear InfirmaryHarvard Medical School, Boston, MA, USA

    Eric Pierce

  3. Health Sciences Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Robert E. Anderson

  4. Department of Ophthalmology, Duke Medical Center, Durham, NC, USA

    Catherine Bowes Rickman

  5. Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

    Joe G. Hollyfield

  6. Laboratory for Retinal Cell BiologyDepartment of OphthalmologyUniversity Hospital Zurich, University of Zurich, Schlieren, Switzerland

    Christian Grimm

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Anderson, D.M.G. et al. (2023). High-Resolution Imaging Mass Spectrometry of Human Donor Eye: Photoreceptors Cells and Basal Laminar Deposit of Age-Related Macular Degeneration. In: Ash, J.D., Pierce, E., Anderson, R.E., Bowes Rickman, C., Hollyfield, J.G., Grimm, C. (eds) Retinal Degenerative Diseases XIX. Advances in Experimental Medicine and Biology, vol 1415. Springer, Cham. https://doi.org/10.1007/978-3-031-27681-1_1

Download citation

Publish with us

Policies and ethics

Search

Navigation