Abstract
Previous studies of retinal pigment epithelium (RPE) morphology found cell-level and spatial patterning differences in many quantitative metrics in comparing normal and disease conditions. However, most of these studies examined eyes from deceased animals. Here we sought to compare noninvasively imaged RPE cells from live mice to histopathology. We describe changes to improve noninvasive imaging of RPE in the live mouse. In retinal diseases, there can be invasion by Iba1-positive cells, which can be detected by noninvasive imaging techniques. Here we can detect potential Iba1-positive cells at the level of the RPE noninvasively.
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Acknowledgments
We are grateful for support from NIH R01EY028450, R01EY021592, P30EY006360, F31EY028855, R01EY028859, T32EY07092, T32GM008490, the Abraham and Phyllis Katz Foundation, VA RR&D I01RX002806 and I21RX001924, VA RR&D C9246C (Atlanta Veterans Administration Center of Excellence in Vision and Neurocognitive Rehabilitation), and an unrestricted grant to the Department of Ophthalmology at Emory University from Research to Prevent Blindness, Inc.
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Donaldson, K.J. et al. (2019). Analysis of Damage and Wound Healing in the Retinal Pigmented Epithelium. In: Bowes Rickman, C., Grimm, C., Anderson, R., Ash, J., LaVail, M., Hollyfield, J. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 1185. Springer, Cham. https://doi.org/10.1007/978-3-030-27378-1_70
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DOI: https://doi.org/10.1007/978-3-030-27378-1_70
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