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Recent Applications of Mass Spectrometry at Clarkson University

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1140))

Abstract

Mass spectrometry (MS) is a powerful technique that has various applications including the identification and characterization of proteins, protein-protein interactions and protein post translational modifications, as well as other molecules (i.e. metabolites, lipids, nucleotides and polynucleotides). However, not too many undergraduate students within the USA and around the world have access to (and are trained in) MS. The undergraduate students in our department are taught to analyze proteomics and metabolomics data obtained from MS analysis, including de novo sequencing of peptides and to interpret the MS and MS/MS data acquired in positive and negative ionization modes. Here, we give some examples of MS data analyzed in the Biochemistry I class and then examples of some independent research projects performed by students over the years in the Biochemistry and Biotechnology laboratory, where MS is used for both proteins, peptides and metabolites analysis, thus demonstrating the applicability of MS analysis in diverse fields. The projects discussed include analysis of the protein content present in yogurt, beer, protein shakes, contact lenses, or milk of animal or vegetal origin.

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Abbreviations

BCA:

Bicinchoninic acid protein assay

ESI:

Electron spray ionization

MALDI:

Matrix assisted laser desorption ionization

MS:

Mass spectrometry

Pkl:

Peaklist

PLGS:

Protein Lynx Global Server

PMFA:

Proteins migrating faster than albumin

QTof:

Quadruple time of flight

SDS-PAGE:

Sodium dodecyl sulfate-poly acrylamide gel electrophoresis

TIC:

Total ion chromatogram

UPLC:

Ultra performance liquid chromatography

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Acknowledgement

We would like to thank all the students, who did their independent research projects in Biochemistry and Biotechnology lab course using MS method for analysis and other students in the lab for creating a pleasant environment. Part of this chapter was partial credit for an extra credit assignment for the Biochemistry I class.

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Authors and Affiliations

  1. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Madhuri Jayathirtha, Danielle Whitham, Sydney Stradtman & Costel C. Darie

Authors
  1. Madhuri Jayathirtha
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  2. Danielle Whitham
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  3. Sydney Stradtman
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  4. Costel C. Darie
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Corresponding author

Correspondence to Costel C. Darie .

Editor information

Editors and Affiliations

  1. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Alisa G. Woods

  2. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Costel C. Darie

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Jayathirtha, M., Whitham, D., Stradtman, S., Darie, C.C. (2019). Recent Applications of Mass Spectrometry at Clarkson University. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 1140. Springer, Cham. https://doi.org/10.1007/978-3-030-15950-4_46

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