Abstract
Splicing components play an essential role in mediating accurate and efficient splicing. The complexity of the spliceosome and its regulatory networks increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory components to carry out intron removal in vitro. This chapter describes the large-scale preparation of nuclear extract from HeLa cells.
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Acknowledgement
Research in the Hertel laboratory is supported by NIH (RO1 GM62287 and R21 CA149548).
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Webb, CH.T., Hertel, K.J. (2014). Preparation of Splicing Competent Nuclear Extracts. In: Hertel, K. (eds) Spliceosomal Pre-mRNA Splicing. Methods in Molecular Biology, vol 1126. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-980-2_8
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DOI: https://doi.org/10.1007/978-1-62703-980-2_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-979-6
Online ISBN: 978-1-62703-980-2
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