Abstract
Behavioral testing methods are described for determining whether female mice can discriminate between volatile urinary pheromones of conspecifics of the same vs. opposite sex and/or in different endocrine conditions, for determining sexual partner preference, for quantifying receptive (lordosis) behavior, and for monitoring the expression of male-typical mounting behavior in female mice.
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References
Baum MJ, Keverne EB (2002) Sex difference in attraction thresholds for volatile odors from male and estrous female mouse urine. Horm Behav 41:213–219
Pierman S, Douhard Q, Balthazart J, Baum MJ, Bakker J (2006) Attraction thresholds and sex discrimination of urinary odorants in male and female aromatase knockout (ArKO) mice. Horm Behav 49:96–104
Brock O, Bakker J (2011) Potential contribution of prenatal estrogens to the sexual differentiation of mate preferences in mice. Horm Behav 59:83–89
Brock O, Baum MJ, Bakker J (2011) The development of female sexual behavior requires prepubertal estradiol. J Neurosci 31:5574–5578
Bakker J, Honda S, Harada N, Balthazart J (2002) The aromatase knock-out mouse provides new evidence that estradiol is required during development in the female for the expression of sociosexual behaviors in adulthood. J Neurosci 22:9104–9112
Mani SK, Reyna AM, Chen JZ, Mulac-Jericevic B, Conneely OM (2006) Differential response of progesterone receptor isoforms in hormone-dependent and -independent facilitation of female sexual receptivity. Mol Endocrinol 20:1322–1332
Ogawa S, Chan J, Chester AE, Gustafsson JA, Korach KS, Pfaff DW (1999) Survival of reproductive behaviors in estrogen receptor beta gene- deficient (betaERKO) male and female mice. Proc Natl Acad Sci U S A 96:12887–12892
Ogawa S, Eng V, Taylor J, Lubahn DB, Korach KS, Pfaff DW (1998) Roles of estrogen receptor-alpha gene expression in reproduction- related behaviors in female mice. Endocrinology 139:5070–5081
Rissman EF, Early AH, Taylor JA, Korach KS, Lubahn DB (1997) Estrogen receptors are essential for female sexual receptivity. Endocrinology 138:507–510
Martel KL, Baum MJ (2009) Adult testosterone treatment but not surgical disruption of vomeronasal function augments male-typical sexual behavior in female mice. J Neurosci 29:7658–7666
Smith ER, Damassa DA, Davidson JM (1977) Plasma testosterone and sexual behavior following intracerebral implantation of testosterone propionate in the castrated male rat. Horm Behav 8:77–87
Wersinger SR, Haisenleder DJ, Lubahn DB, Rissman EF (1999) Steroid feedback on gonadotropin release and pituitary gonadotropin subunit mRNA in mice lacking a functional estrogen receptor alpha. Endocrine 11:137–143
Keller M, Douhard Q, Baum MJ, Bakker J (2006) Destruction of the main olfactory epithelium reduces female sexual behavior and olfactory investigation in female mice. Chem Senses 31:315–323
Pankevich DE, Cherry JA, Baum MJ (2006) Effect of vomeronasal organ removal from male mice on their preference for and neural Fos responses to female urinary odors. Behav Neurosci 120:925–936
Sorwell KG, Wesson DW, Baum MJ (2008) Sexually dimorphic enhancement by estradiol of male urinary odor detection thresholds in mice. Behav Neurosci 122:788–793
Wesson DW, Keller M, Douhard Q, Baum MJ, Bakker J (2006) Enhanced urinary odor discrimination in female aromatase knockout (ArKO) mice. Horm Behav 49:580–586
Acknowledgment
Preparation of this review was supported, in part, by FRSM grant 3.4571.10 to J.B.
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Brock, O., Bakker, J., Baum, M.J. (2013). Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice. In: Touhara, K. (eds) Pheromone Signaling. Methods in Molecular Biology, vol 1068. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-619-1_24
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DOI: https://doi.org/10.1007/978-1-62703-619-1_24
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Publisher Name: Humana Press, Totowa, NJ
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