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Immobilization of Enzymes on Monofunctional and Heterofunctional Epoxy-Activated Supports

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Immobilization of Enzymes and Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1051))

Abstract

The immobilization of proteins on epoxy activated supports is discussed in this chapter. Immobilization on epoxy supports is carried out as a two-step mechanism: in the first step the adsorption of the protein is promoted and in the second step the intramolecular covalent linkage among epoxy groups and nucleophiles of the protein is produced. Based on this mechanism of the need of a first adsorption of the protein on the support, different epoxy supports are described. The different supports are able to immobilize proteins through different orientations being obtained catalysts with different properties of activity, stability, and selectivity.

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Author information

Authors and Affiliations

  1. Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain

    Cesar Mateo

  2. Nanotherapy and Nanodiagnostics Group (GN2), Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Zaragoza, Spain

    Valeria Grazu

  3. Instituto de Catalisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

Authors
  1. Cesar Mateo
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  2. Valeria Grazu
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  3. Jose M. Guisan
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Editor information

Editors and Affiliations

  1. Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

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Mateo, C., Grazu, V., Guisan, J.M. (2013). Immobilization of Enzymes on Monofunctional and Heterofunctional Epoxy-Activated Supports. In: Guisan, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 1051. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-550-7_4

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  • DOI: https://doi.org/10.1007/978-1-62703-550-7_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-549-1

  • Online ISBN: 978-1-62703-550-7

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