Abstract
Small interfering RNA (siRNA) has the potential to influence gene expression with a high degree of target gene specificity. However, the clinical application of siRNA therapeutics has proven to be less promising as evidenced by its poor intracellular uptake, instability in vivo, and nonspecific immune stimulations. Recently, we have demonstrated that single-walled carbon nanotube (SWNT)-mediated siRNA delivery can enhance the efficiency of siRNA-mediated gastrin-releasing peptide receptor (GRP-R) gene silencing by stabilizing siRNA while selectively targeting tumor tissues. Based on our recent findings, we introduce a novel technique to silence specific gene(s) in human neuroblastoma through SWNT-mediated siRNA delivery in vitro and in vivo.
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Acknowledgments
The authors would like to thank Nadja Colon and Roel L. Flores for critical review of the manuscript. This work was supported by R01 DK61470 (DHC) from the National Institutes of Health and ECCS-1055852 from National Science Foundation (YQX).
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Qiao, J., Hong, T., Guo, H., Xu, YQ., Chung, D.H. (2013). Single-Walled Carbon Nanotube-Mediated Small Interfering RNA Delivery for Gastrin-Releasing Peptide Receptor Silencing in Human Neuroblastoma. In: Rosenthal, S., Wright, D. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology, vol 1026. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-468-5_11
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DOI: https://doi.org/10.1007/978-1-62703-468-5_11
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