Abstract
Osmotic stress induces several senescence-like processes in leaves, such as specific changes in gene expression and yellowing. These processes are dependent on the accumulation of jasmonates and on intact jasmonate signaling. This chapter describes the treatment of Arabidopsis thaliana leaves with sorbitol as an osmotic stress agent and the determination of the elicited phenotypes encompassing chlorophyll loss, degradation of plastidial membrane lipids, and induction of genes regulated by senescence and jasmonate.
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Acknowledgments
We would like to acknowledge the help of Nadja Stingl and Martin J. Mueller. This work was supported by the SFB567 and the GK1342 of the “Deutsche Forschungsgemeinschaft.”
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Seltmann, M.A., Berger, S. (2013). Phenotyping Jasmonate Regulation of Senescence. In: Goossens, A., Pauwels, L. (eds) Jasmonate Signaling. Methods in Molecular Biology, vol 1011. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-414-2_1
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DOI: https://doi.org/10.1007/978-1-62703-414-2_1
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Publisher Name: Humana Press, Totowa, NJ
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