Abstract
To use Caenorhabditis elegans to study the mechanisms for initiation and execution of necrosis, the experimentalist should be familiar with the established models of necrosis in C. elegans and the genetic and molecular tools available. We present a summary of two contrasting models for studying necrosis in C. elegans and outline the methods for scoring necrosis in each. These methods are useful for the study of necrosis under other conditions in C. elegans and for comparative studies both between established and new necrosis models. We also present a list of the genetic and drug tools available for perturbing pathways known to be important for initiation or execution of necrosis and describe their use in C. elegans. Specifically, we outline methods to inhibit autophagy, to perturb calcium homeostasis, and to disrupt lysosomal function in the C. elegans system.
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References
Golstein P, Kroemer G (2007) Cell death by necrosis: towards a molecular definition. Trends Biochem Sci 32:37–43
McCall K (2010) Genetic control of necrosis—another type of programmed cell death. Curr Opin Cell Biol 22:882–888
Syntichaki P, Tavernarakis N (2002) Death by necrosis. Uncontrollable catastrophe, or is there order behind the chaos? EMBO Rep 3:604–609
Chalfie M, Wolinsky E (1990) The identification and suppression of inherited neurodegeneration in Caenorhabditis elegans. Nature 345:410–416
Hall DH, Gu G, Garcia-Anoveros J et al (1997) Neuropathology of degenerative cell death in Caenorhabditis elegans. J Neurosci 17:1033–1045
Driscoll M, Chalfie M (1991) The mec-4 gene is a member of a family of Caenorhabditis elegans genes that can mutate to induce neuronal degeneration. Nature 349:588–593
Hall DH, Altun ZF (2008) C. elegans atlas. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Chalfie M, Sulston J (1981) Developmental genetics of the mechanosensory neurons of Caenorhabditis elegans. Dev Biol 82:358–370
Syntichaki P, Xu K, Driscoll M et al (2002) Specific aspartyl and calpain proteases are required for neurodegeneration in C. elegans. Nature 419:939–944
Xu K, Tavernarakis N, Driscoll M (2001) Necrotic cell death in C. elegans requires the function of calreticulin and regulators of Ca(2+) release from the endoplasmic reticulum. Neuron 31:957–971
Syntichaki P, Samara C, Tavernarakis N (2005) The vacuolar H+-ATPase mediates intracellular acidification required for neurodegeneration in C. elegans. Curr Biol 15:1249–1254
Artal-Sanz M, Samara C, Syntichaki P et al (2006) Lysosomal biogenesis and function is critical for necrotic cell death in Caenorhabditis elegans. J Cell Biol 173:231–239
Samara C, Syntichaki P, Tavernarakis N (2008) Autophagy is required for necrotic cell death in Caenorhabditis elegans. Cell Death Differ 15:105–112
Toth ML, Simon P, Kovacs AL et al (2007) Influence of autophagy genes on ion-channel-dependent neuronal degeneration in Caenorhabditis elegans. J Cell Sci 120:1134–1141
Huang L, Hanna-Rose W (2006) EGF signaling overcomes a uterine cell death associated with temporal mis-coordination of organogenesis within the C. elegans egg-laying apparatus. Dev Biol 300:599–611
Vrablik TL, Huang L, Lange SE et al (2009) Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival in C. elegans. Development 136:3637–3646
Strange K (2006) C. elegans: methods and applications. Humana, Totowa, NJ
Hope IA (1999) C. elegans: a practical approach. Oxford University Press, Oxford, NY
Pivtoraiko VN, Harrington AJ, Mader BJ et al (2010) Low-dose bafilomycin attenuates neuronal cell death associated with autophagy-lysosome pathway dysfunction. J Neurochem 114:1193–1204
Acknowledgement
This work was supported by NIH grant GM086786 to W.H.R.
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Crook, M., Upadhyay, A., Hanna-Rose, W. (2013). Necrosis in C. elegans. In: McCall, K., Klein, C. (eds) Necrosis. Methods in Molecular Biology, vol 1004. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-383-1_13
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DOI: https://doi.org/10.1007/978-1-62703-383-1_13
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