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Self-Assembling Nanomaterials: Monitoring the Formation of Amyloid Fibrils, with a Focus on Small-Angle X-Ray Scattering

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Book cover Protein Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 996))

Abstract

Amyloid fibrils are attractive targets for applications in biotechnology. These thin, nanoscale protein fibers are highly ordered structures that self-assemble from their component proteins or peptides. This chapter describes the use of several biophysical techniques to monitor the formation of amyloid fibrils including a common dye-binding assay, turbidity assay, and small-angle X-ray scattering. These techniques provide information about the assembly mechanism, the rate and reproducibility of assembly, as well as the size of species along the assembly pathway.

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Author information

Authors and Affiliations

  1. Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Australia

    Elizabeth B. Sawyer & Sally L. Gras

  2. Department of Chemical and Biomolecular Engineering, The University of Melbourne, Melbourne, Australia

    Elizabeth B. Sawyer & Sally L. Gras

Authors
  1. Elizabeth B. Sawyer
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  2. Sally L. Gras
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Editor information

Editors and Affiliations

  1. University of Canterbury, Kirkwood Avenue 20, Christchurch, 8041, New Zealand

    Juliet A. Gerrard

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Sawyer, E.B., Gras, S.L. (2013). Self-Assembling Nanomaterials: Monitoring the Formation of Amyloid Fibrils, with a Focus on Small-Angle X-Ray Scattering. In: Gerrard, J. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 996. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-354-1_5

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  • DOI: https://doi.org/10.1007/978-1-62703-354-1_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-353-4

  • Online ISBN: 978-1-62703-354-1

  • eBook Packages: Springer Protocols

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