Abstract
Several different precursor populations participate in renewal and regeneration of the mammalian skin and hair follicle. Recently, we described the existence of multipotent dermal precursors that exhibit properties of stem cells, and reside in the mesenchymal compartment of the hair follicle. When isolated and grown in vitro, these cells give rise to self-renewing, multipotent, spherical colonies of cells called Skin-derived Precursors (or “SkPs”). Here we describe methods to isolate SkPs from rodent and human skin and provide assays to determine functional differentiation of their progeny.
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Acknowledgments
We thank Mr. Ranjan Kumar for his assistance with images. This work was funded by a CIHR operating grant and a CIHR New Investigator Award to J.B.
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Hagner, A., Biernaskie, J. (2013). Isolation and Differentiation of Hair Follicle-Derived Dermal Precursors. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 989. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-330-5_19
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DOI: https://doi.org/10.1007/978-1-62703-330-5_19
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-330-5
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