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Setting Up and Monitoring an Infection of Dictyostelium discoideum with Mycobacteria

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Dictyostelium discoideum Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 983))

Abstract

Mycobacterium marinum is the causative agent of fish and amphibian tuberculosis in the wild. It is a genetically close cousin of Mycobacterium tuberculosis, and thereby the infection process remarkably shares many of the hallmarks of M. tuberculosis infection in human, at both the cellular and organism levels. Therefore, M. marinum is used as a model for the study of mycobacterial infection in various host organisms. Recently, the Dictyostelium–M. marinum system has been shown to be a valuable model that recapitulates the main features of the intracellular fate of M. marinum including phagosome maturation arrest, as well as its particular cell-to-cell dissemination mode. We present here a “starter kit” of detailed methods that allows to establish an infection of Dictyostelium with M. marinum and to monitor quantitatively the intracellular bacterial growth.

*onia Arafah and Sébastien Kicka contributed equally to the work.

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Acknowledgement

The laboratory of Thierry Soldati is supported by multiple grants from the Swiss National Science Foundation.

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Authors and Affiliations

  1. Départment de Biochimie, Faculté des Sciences, Université de Genève, Geneva, Switzerland

    Sonia Arafah, Sébastien Kicka, Valentin Trofimov & Thierry Soldati

  2. Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany

    Monica Hagedorn

  3. Department of Medicine, Centre for Molecular Microbiology and Infection, Imperial College London, London, UK

    Nuria Andreu

  4. Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand

    Siouxsie Wiles & Brian Robertson

Authors
  1. Sonia Arafah
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  2. Sébastien Kicka
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  3. Valentin Trofimov
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  4. Monica Hagedorn
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  5. Nuria Andreu
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  6. Siouxsie Wiles
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  7. Brian Robertson
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  8. Thierry Soldati
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Corresponding author

Correspondence to Thierry Soldati .

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Editors and Affiliations

  1. , Centre for Biochemistry, University of Cologne, Joseph-Stelzmann-Str. 52, Köln, 50931, Nordrhein-Westfalen, Germany

    Ludwig Eichinger

  2. , Centre for Biomedical Research, University of Hull, Hull, HU6 7RX, Montserrat

    Francisco Rivero

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Arafah, S. et al. (2013). Setting Up and Monitoring an Infection of Dictyostelium discoideum with Mycobacteria. In: Eichinger, L., Rivero, F. (eds) Dictyostelium discoideum Protocols. Methods in Molecular Biology, vol 983. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-302-2_22

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  • DOI: https://doi.org/10.1007/978-1-62703-302-2_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-301-5

  • Online ISBN: 978-1-62703-302-2

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