Abstract
Given the complexity of morphological presentation and variability in clinical outcomes observed in epithelial cancers, it is important to understand how genomic perturbations and resultant molecular aberrations lead to acquisition of tumorigenic phenotypes. Complex 3D epithelial culture systems provide investigators with the ability to propagate and manipulate primary cells in an appropriate physical setting in order to deconstruct the contribution of a given genetic lesion(s) to the process of cellular transformation. Pancreatic ductal epithelial cells (PDEC) can give rise to pancreatic intraepithelial neoplasia—precursor lesions that precede pancreatic ductal adenocarcinoma (PDA). In this chapter, we describe a series of methods for derivation and culture of primary PDEC, which can be used to elucidate the mechanistic contribution of oncogenic insults to the initiation and progression of pancreatic tumorigenesis.
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Acknowledgments
We thank L.J. Taylor for help with manuscript preparation. This work was supported by the National Institutes of Health Grant CA055360, AACR-PanCAN grant 08-60-25-BARS (both to D.B-S.) and by The Irvington Institute Fellowship Program of the Cancer Research Institute (Y.P-G.)
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Pylayeva-Gupta, Y., Lee, K.E., Bar-Sagi, D. (2013). Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_14
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DOI: https://doi.org/10.1007/978-1-62703-287-2_14
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