Abstract
Virus-induced gene silencing (VIGS) is an effective tool for the analysis of the gene function in plants within a short time. However, in woody fruit tree like apple, some of Solanum crops, and soybean, it is generally difficult to inoculate virus vector by conventional inoculation methods. Here, we show efficient VIGS in apple and soybean by Apple latent spherical virus (ALSV) vector and biolistic inoculation. The plants inoculated with ALSV vectors by particle bombardment showed uniform silenced phenotypes of target genes within 2–3 weeks post inoculation.
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Acknowledgements
We thank S. Sasaki for his technical support. This work was supported in part by Grant-in-Aids for Research and Development Projects for Application in Promoting New Policy of Agriculture, Forestry and Fisheries from the Ministry of Agriculture, Forestry and Fisheries, KAKENHI (no. 20380025) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry.
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Yamagishi, N., Yoshikawa, N. (2013). Highly Efficient Virus-Induced Gene Silencing in Apple and Soybean by Apple Latent Spherical Virus Vector and Biolistic Inoculation. In: Becker, A. (eds) Virus-Induced Gene Silencing. Methods in Molecular Biology, vol 975. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-278-0_13
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DOI: https://doi.org/10.1007/978-1-62703-278-0_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-277-3
Online ISBN: 978-1-62703-278-0
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