Abstract
The ability of B lymphocytes to capture external antigens (Ag) and present them as peptide fragments, loaded on Major Histocompatibility complex (MHC) class II molecules, to CD4+ T cells is a crucial part of the adaptive immune response. This allows T-B cooperation, a cellular communication that is required for B cells to develop into germinal centers (GC) and form mature high-affinity antibody producing cells and to further develop B cell memory. MHC class II antigen presentation by B lymphocytes is a multistep process involving (1) Recognition and capture of external Ag by B lymphocytes through their B cell receptor (BCR); (2) Ag processing, which comprises the degradation of Ag in internal compartments within the B cell and loading of the corresponding peptide fragments on MHC class II molecules and (3) Presentation of MHCII-peptide complexes to CD4+ T cells. Here, we describe how to study MHC class II antigen presentation by B lymphocytes at these three major levels.
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Abbreviations
- Ag:
-
Antigen
- BCR:
-
B cell receptor
- BSA:
-
Bovine serum albumin
- FBS:
-
Fetal bovine serum
- GC:
-
Germinal center
- Ig:
-
Immunoglobulin
- IS:
-
Immune synapse
- MHC:
-
Major histocompatibility complex
- MTOC:
-
Microtubule organizing center
- OVA:
-
Ovalbumin
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Yuseff, M.I., Lennon-Dumenil, A.M. (2013). Studying MHC class II Presentation of Immobilized Antigen by B Lymphocytes. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_39
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DOI: https://doi.org/10.1007/978-1-62703-218-6_39
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