Abstract
Caspase-1 is a critical factor in the innate immune response to Legionella pneumophila. The development of methods for analyzing caspase-1 activation pathways and downstream caspase-1-associated activities has helped in understanding the regulation of this protease and the signaling components involved. Here we outline methods for directly detecting active caspase-1, measuring caspase-1 activities and analyzing components involved in the regulation of caspase-1 during L. pneumophila infection in macrophages.
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Case, C.L., Roy, C.R. (2013). Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_29
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DOI: https://doi.org/10.1007/978-1-62703-161-5_29
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-160-8
Online ISBN: 978-1-62703-161-5
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