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Nanoimaging pp 359–371Cite as

Functional AFM Imaging of Cellular Membranes Using Functionalized Tips

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 950))

Abstract

The real-time visualization of specific binding sites on biological samples with high spatial resolution, in order of several nanometers, is an important undertaking in many fields of biology. During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful tool to quickly obtain local receptor nanomaps on complex heterogeneous biosurfaces, such as cells and membranes. In this chapter, we present the TREC technique and explain how to unravel the nano-landscape of cells of the immune system, such as macrophages. We describe the procedures for all steps of the experiment including tip functionalization with Fc fragments via flexible PEG-linker, sample preparation, and localization of Fcγ receptors on macrophages.

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Author information

Authors and Affiliations

  1. Center for Advanced Bioanalysis GmbH (CBL), Linz, Austria

    Lilia A. Chtcheglova

  2. Institute for Biophysics, University of Linz, Linz, Austria

    Peter Hinterdorfer

Authors
  1. Lilia A. Chtcheglova
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  2. Peter Hinterdorfer
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Corresponding author

Correspondence to Lilia A. Chtcheglova .

Editor information

Editors and Affiliations

  1. Nat. Inst. Biomedical Imaging & Bioengin, National Institutes of Health, South Drive 13, Bethesda, 20892, Maryland, USA

    Alioscka A. Sousa

  2. , National Cancer Institute, National Institutes of Health, Center Drive 10, Bethesda, 20892, USA

    Michael J. Kruhlak

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Chtcheglova, L.A., Hinterdorfer, P. (2013). Functional AFM Imaging of Cellular Membranes Using Functionalized Tips. In: Sousa, A., Kruhlak, M. (eds) Nanoimaging. Methods in Molecular Biology, vol 950. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-137-0_20

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  • DOI: https://doi.org/10.1007/978-1-62703-137-0_20

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-136-3

  • Online ISBN: 978-1-62703-137-0

  • eBook Packages: Springer Protocols

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